基于生物信息学识别和验证与冠状动脉疾病患者的衰老相关的枢纽基因
Wangmeng Zhang1, Minmin Zhao1, Li Xin2
1Department of Obstetrics, The Affiliated Tai’an City Central Hospital of Qingdao University, Tai’an 271000, Shandong, China.
Aging
|December 14, 2023
概括
冠状动脉疾病 (CAD) 与衰老有关. 生物信息学确定HSP90AA1和CEBPA是与衰老和CAD相关的关键基因,表明它们作为诊断和治疗生物标志物的潜力.
科学领域:
- 基因组学就是基因组学.
- 衰老研究研究 衰老研究
- 心血管疾病 心血管疾病
背景情况:
- 冠状动脉疾病 (CAD) 是一个普遍的与衰老相关的疾病.
- 了解衰老和CAD之间的遗传联系对于开发新疗法至关重要.
研究的目的:
- 使用生物信息学识别与衰老和CAD相关的基因.
- 在临床样本中验证潜在的枢纽基因,以诊断和治疗相关性.
主要方法:
- 下载并分析微阵列数据 (GSE12288),以识别CAD相关基因.
- 整合了来自Aging Atlas的与衰老相关的基因的数据,以找到差异表达的与衰老相关的基因 (DEARGs).
- 利用生物信息学工具 (Cytoscape) 和验证数据集 (GSE71226) 来选枢纽基因,然后进行实验验证 (qPCR,ELISA,西斑).
主要成果:
- 鉴定出33种DEARG,富含细胞粘附,免疫细胞分化和寿命调节等途径.
- 选和验证HSP90AA1和CEBPA作为重要的枢纽基因.
- 在临床样本中确认了HSP90AA1和CEBPA在转录和转化水平上的差异表达.
结论:
- HSP90AA1和CEBPA与冠状动脉疾病密切相关.
- 这些基因为衰老和CAD之间的联系提供了理论基础.
- 作为CAD诊断和治疗的生物标志物,HSP90AA1和CEBPA显示出有前途.
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