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相关概念视频

Replication in Prokaryotes01:32

Replication in Prokaryotes

24.9K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
24.9K
Replication in Eukaryotes01:29

Replication in Eukaryotes

13.8K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
13.8K
Chromosome Replication02:31

Chromosome Replication

8.7K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
8.7K
The DNA Replication Fork01:02

The DNA Replication Fork

36.0K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
36.0K
The Replisome03:01

The Replisome

33.5K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
33.5K
Chromosome Structure02:40

Chromosome Structure

22.8K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
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相关实验视频

Updated: Jul 8, 2025

Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
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Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

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细菌的复制起源BUS促进了核基捕获.

Simone Pelliciari1, Salomé Bodet-Lefèvre2, Stepan Fenyk1

  • 1Centre for Bacterial Cell Biology, Biosciences Institute, Newcastle University, Newcastle Upon Tyne, NE2 4AX, UK.

Nature communications
|December 14, 2023
PubMed
概括
此摘要是机器生成的。

细菌DNA复制启动蛋白DnaA (腺三酸盐) 组装成一个复合体,打开染色体起源. 这种分子机制解释了DnaA如何结合DNA链来启动基因组复制.

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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

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Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
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Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

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相关实验视频

Last Updated: Jul 8, 2025

Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
11:12

Determination of the Optimal Chromosomal Locations for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

Published on: September 11, 2017

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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

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Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
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Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物化学 生化学

背景情况:

  • 基因组复制对于细胞增殖至关重要,通常由染色体起源的复制蛋白开始.
  • 在细菌中,DNA复制始于DnaA蛋白,该蛋白在复制的起源 (oriC) 形成一个寡合体复合体.
  • 这种DnaA-oriC复合体涉及双链DNA (dsDNA) 和单链DNA (ssDNA),以促进DNA双重开放,但确切的机制尚不清楚.

研究的目的:

  • 阐明DnaA专门打开细菌复制起源的分子机制.
  • 了解DnaA如何与oriC的DNA相互作用以启动复制.

主要方法:

  • 在DNA化场所研究了Bacillus subtilis DnaAATP的组装.
  • 在复合体内分析了DnaA与dsDNA和ssDNA的相互作用.
  • 描述了在DnaA寡合体内ssDNA的结合.

主要成果:

  • 细菌细菌DnaAATP在DNA融化部位形成一个连续的寡合体,桥接dsDNA和ssDNA.
  • DnaA寡合体从dsDNA延伸到单一的DNA链.
  • 每个ssDNA结合基因 (DnaA-trio) 在由相邻的DnaA蛋白形成的二核酸结合口袋中捕获两个核基.

结论:

  • 这项研究为DnaA如何启动DNA复制起源开放提供了分子解释.
  • 这些发现揭示了DNAA在细菌起源的基底解系统 (BUS) 中引入保存序列元素的特定机制.
  • 这说明了细菌DNA复制启动的一个基本步骤.