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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
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用RNASeq数据识别人类结直肠癌中差异表达的基因,这些数据在分子水平上通过实时PCR验证.

Aya M A Elsayed1,2,3, Mariam Oweda4, Asmaa M Abushady1,5

  • 1School of Biotechnology, Nile University, Giza, Egypt.

Biochemical genetics
|December 15, 2023
PubMed
概括

这项研究确定了用于早期结直肠癌 (CRC) 检测的新型基因生物标志物,并探索了基于植物的治疗方法. 这些发现突出了CRC的潜在诊断标志物和治疗途径,改善了患者的治疗结果.

关键词:
在Withhania somenifra中,有一些问题.结肠直肠癌是一种癌症.DEGs 是一个DEG.在 DESeq2 包装中,地理地质组织 (GEO) 是指地理地质组织.在GProfiler中,我们使用的是GProfiler.在MMP7中,MMP7是MMP7.在RNA-Seqq.在TCF2121中,我们可以看到:在VEGFD中,VEGFD是VEGFD.

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科学领域:

  • 在瘤学瘤学.
  • 生物信息学是一种生物信息学.
  • 分子生物学分子生物学

背景情况:

  • 结肠直肠癌 (CRC) 给全球健康带来了重大挑战,因为晚诊影响了生存率.
  • 确定可靠的生物标志物对于早期检测和改善CRC患者预后至关重要.

研究的目的:

  • 为确定结直肠癌 (CRC) 的诊断和预后生物标志物开发生物信息学管道.
  • 研究植物提取物的潜力,作为CRC治疗化学疗法的替代或辅助.
  • 用分子和体外方法验证已识别的生物标志物和治疗点.

主要方法:

  • 利用来自癌症和正常组织的RNA-seq数据,使用FastQC,Kallisto和DESeq2等工具进行处理.
  • 使用生物信息学工具,包括g:Profiler,GNEMANIA-CytoScape和CytoHubba用于基因丰富和网络分析.
  • 进行了体外试验,使用5-甲和Withhania somnifera提取物,并进行了分子对接研究.

主要成果:

  • 在CRC中鉴定了1641个差异表达基因 (DEG),富含各种信号通路.
  • MMP7,TCF21和VEGFD已成为结直肠癌的有前途的诊断生物标志物.
  • 维塔尼亚桑尼菲拉提取物在基因表达和潜在的治疗价值方面显示出积极的趋势,向关键基因.

结论:

  • 该研究提出了一个强大的生物信息工作流程,用于识别DEG作为潜在的CRC生物标志物.
  • MMP7,TCF21和VEGFD被强调为早期CRC检测的重要诊断标记.
  • 维坦尼亚·索姆尼费拉提取物在CRC中显示出治疗潜力,因此需要进一步研究甲基化促进剂调节.