在群体内具有相反的流行病学行为的金黄色葡萄球菌菌株的分泌体会影响牛单核细胞反应
Susanna Di Mauro1, Joel Filipe1, Alessia Facchin1
1Department of Veterinary Medicine and Animal Science, Università degli Studi di Milano, via dell'Università 6, 26900, Lodi, Italy.
Veterinary research
|December 15, 2023
概括
不同菌株的黄金葡萄球菌分泌物对牛免疫细胞的影响不同. 一种菌株提高了细胞活力和炎症,另一种菌株损害了细胞活力.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 兽医科学 兽医科学 兽医科学
背景情况:
- 黄金葡萄球菌的分泌物表现出特定菌株的毒性机制.
- 由S. aureus调节宿主免疫反应涉及直接的细胞相互作用和分泌的分子.
- 黄金杆菌菌株的流行病学行为与明显的分泌物概况相关.
研究的目的:
- 调查来自两个不同的菌株的金黄色细菌分泌体对外围牛单核细胞 (PBMCs) 的影响.
- 为了比较来自高发病率菌株 (GTB/ST8) 和低发病率菌株 (GTS/ST398) 的秘密体对PBMC活力和免疫基因表达的影响.
主要方法:
- 在不同度 (0-2.5%) 的GTB/ST8和GTS/ST398分泌体中,PBMC被化了18小时和48小时.
- 细胞活力是使用标准测试来评估的.
- 通过RT-qPCR量化了促炎 (IL1-β,STAT1) 和抗炎 (IL-10,STAT6,TGF-β) 基因的mRNA水平,以及特定的miRNAs (miR-155-5p,miR-125b-5p,miR-146a,miR-145).
主要成果:
- 在2.5%的GTB/ST8秘密基因增加了PBMC的活力.
- 在2.5%的GTS/ST398秘密原子显著降低了PBMC的生存能力,停止了进一步的分析.
- 在没有影响抗炎标志物的情况下,GTB/ST8秘密基因诱导了亲炎性PBMC反应,对IL1-β,STAT1和miR-155-5p进行上调.
结论:
- 具有不同流行病学行为的金黄色菌株对牛免疫细胞产生不同的影响.
- GTS/ST398秘密体对PBMCs具有细胞毒性,而GTB/ST8秘密体提高了细胞活力,并促进了亲炎性免疫表型.
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