在早期新陈代谢中单一的酸化机制 - - 聚酸的例子
Joris Zimmermann1, Robert J Mayer1, Joseph Moran1,2,3
1Institut de Science et d'Ingénierie Supramoléculaires (ISIS), CNRS UMR 7006, Université de Strasbourg 8 Allée Gaspard Monge 67000 Strasbourg France.
Chemical science
|December 15, 2023
概括
研究人员探索了一种祖先的酸化机制,用于将pyruvate转化为phosphoenolpyruvate (PEP). 他们发现,碳酸盐酸化,而不是乙烯酸盐,很可能启动了这个关键的代谢反应,这表明自我组织的化学起源.
科学领域:
- 生物化学 生物化学
- 代谢的起源 代谢的起源
- 化学进化的化学进化
背景情况:
- 酸化对于代谢的出现至关重要.
- 大多数微生物的代谢酸化作用于碳酸盐.
- 酸盐对酸 (PEP) 酸化是一种例外,涉及酸盐.
研究的目的:
- 调查祖先的酸盐酸化是否使用碳酸盐酸化.
- 探索早期代谢途径出现的化学可信性.
主要方法:
- 使用P4O10作为模型酸化剂.
- 使用模型基板进行机械学研究.
- 使用时间过程实验,NMR光谱 (液态和固态) 和DFT计算.
主要成果:
- 通过碳酸盐酸化发生了与P4O10的酸盐酸化.
- 形成了一种酸乙烯中间体,并经历了分子内酸转移.
- 这种机制成功地产生了PEP.
结论:
- 一个单一的碳酸盐酸化机制可以启动包括PEP在内的代谢网络.
- 这支持了新陈代谢从自我组织的化学物质中出现的假设.
- 挑战了PEP形成的酸盐特异性祖先机制的概念.
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