化物提高了化效率,并在同时化和脱化过程中丰富了氨氧化古生物和细菌
Yu Xiang1,2, Tengzhi Zhou2, Siping Deng2
1School of Architecture and Civil Engineering, Xihua University, Chengdu 610039, China.
将酸盐添加到同时化和脱化 (SND) 废水处理中,可以显著提高氨去除效率. 这一过程增强了氧化氨的古生物和细菌,改善了整体的去除.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 废水处理 废水处理
背景情况:
- 同时化和脱 (SND) 是一种高效,节能的废水处理方法.
- 化物是SND中的中间体,影响氨氧化效率和化器社区结构.
研究的目的:
- 研究酸盐添加对SND反应堆中氨氧化效率的影响.
- 分析酸盐对氨氧化古生物 (AOA) 和氨氧化细菌 (AOB) 种群的影响.
主要方法:
- 两个SND反应堆运行:一个没有酸盐的控制反应堆 (R1) 和一个添加20毫克N/L酸盐的实验反应堆 (R2).
- 测量了氨的去除速度,并使用特定的抑制剂确定了AOA和AOB的贡献.
- 使用定量PCR (qPCR) 和高通量测序来分析AOA和AOB的丰富性和组成,重点关注氨一氧化酶基因 (amoA).
主要成果:
- 总去除效率从R1的74.5%增加到R2.2的99.0%.
- 在R2中,氨的去除率是R1的4.5倍,其中AOA和AOB的贡献很大.
- 化物添加导致AOAamoA副本增加了280倍,AOBamoA副本增加了30倍,丰富了特定的AOB物种.
结论:
- 化物添加对SND过程中的氨去除效率和速率产生积极影响.
- 化物添加丰富了AOA和AOB种群,增强了它们的氨氧化能力.
- 在AOA和AOB中存在特定的酸盐还原酶,有助于适应酸盐的添加.
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