相关实验视频

Updated: Jul 8, 2025

Visualization of Inflammatory Caspases Induced Proximity in Human Monocyte-Derived Macrophages

Visualization of Inflammatory Caspases Induced Proximity in Human Monocyte-Derived Macrophages

Published on: April 6, 2022

刺激C5a会诱导人体外围血液单核细胞中的caspase-1激活和成熟IL-1β的产生

Yuya Fujita¹, Haruki Matsumoto¹, Kenji Inada¹

¹Department of Rheumatology, Fukushima Medical University School of Medicine, Fukushima, Japan.

Immunological medicine

|December 15, 2023

概括

补充成分C5a通过caspase-1激活刺激单细胞中成熟的互白素-1β (IL-1β) 生产. 这一发现澄清了C5a与炎症反应中的炎症酶激活之间的联系.

科学领域:

免疫学免疫学免疫学
炎症研究炎症研究
分子生物学分子生物学

背景情况:

已知补充成分C5a可以将免疫细胞招募到炎症组织中.
益炎性细胞因子介素-1β (IL-1β) 通过炎症酶激活与炎症性疾病有关.
C5a和炎症酶激活之间的确切关联仍然不清楚.

研究的目的:

研究C5a对人类外周血液单核细胞 (PBMCs) 中IL-1β产生的影响.
阐明单细胞和caspase-1在C5a介导IL-1β分泌中的作用.

主要方法:

人类PBMCs被C5a刺激.
使用ELISA测量IL-1β分泌量.
蛋白质表达 (亲IL-1β和切割IL-1β) 被西方博客分析.
卡斯帕斯-1激活被FACS检测到.
用单细胞枯竭和淋巴细胞枯竭的PBMC来确定IL-1β生产的细胞来源.

主要成果:

C5a刺激导致PBMC中的IL-1β的度依赖的产生.
亲IL-1β水平增加,并检测到分裂IL-1β (p17),表明IL-1β成熟.
在C5a刺激的CD14+单细胞中发现了活性caspase-1.
单细胞枯竭的PBMCs,但不是淋巴细胞枯竭的PBMCs,在C5a刺激时产生分裂IL-1β.

结论:

单独C5a可以诱导人类PBMCs中成熟IL-1β的产生.
由C5a触发的IL-1β产生主要由CD14+单细胞内的caspase-1激活介导.
这些发现突出了一个新的机制,C5a通过单细胞中的IL-1β成熟促进炎症.

关键词:

在C5a中,它是C5a.一个IL-1ββ.一些炎症一些炎症单细胞 (Monocytes) 是一种单细胞.

更多相关视频

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

相关概念视频

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

Differentiation of Common Myeloid Progenitor Cells

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...

Inflammation

Inflammation