牛犊床铺的中期储存会影响细菌群体和灭活动物感染细菌的有效性
Delphine Rapp1, Colleen Ross1, Vanessa Cave2
1Food System Integrity, AgResearch Ltd, Hopkirk Research Institute, Palmerston North, New Zealand.
PloS one
|December 15, 2023
概括
在采集后立即储存动物床可以增强大肠杆菌O157:H7.7的失活性. 储存前减少了无活化效果,而Salmonella enterica的无活化在储存时受到的影响较小,这表明床铺管理对动物传染病原体的控制有影响.
科学领域:
- 环境微生物学环境微生物学
- 食品安全 食品安全
- 畜牧业 动物养殖 动物养殖
背景情况:
- 动物废物,包括床上用品,在土地上应用,可以将动物传染病原体引入食品系统.
- 了解床铺材料中的病原体失活对于减轻食品安全风险至关重要.
- 牛污染的床铺造成了传播病原体的风险,如大肠杆菌O157:H7和沙门氏菌.
研究的目的:
- 评估动物床铺在减少大肠杆菌O157:H7和沙门氏菌的有效性.
- 为了确定床铺储存时间对病原体无活化的影响.
- 调查本地细菌社区在床铺材料中的病原体命运中的作用.
主要方法:
- 引入大肠杆菌O157:H7和S. enterica尾酒在高度中进入小牛床材.
- 使用最可能数 (MPN) 方法,在10周内监测病原体无活化.
- 通过16S rRNA基因测序对细菌社区进行分析,以评估社区结构和多样性.
主要成果:
- 与预存2个月的床上用品相比,立即接种床上用品导致大大肠杆菌O157:H7无活化 (6.6Log10g-1减少) 显著增加 (4.9Log10g-1减少).
- 沙门氏菌体表现出较少有效的无活化 (大小减少一级),而未储存和预先储存的床铺之间没有显著差异.
- 储存前改变了床铺细菌群体的组成,但不是多样性;多样化的社区可能有助于病原体的无活化,可能涉及纤维细胞分解性细菌.
结论:
- 动物床铺储存实践显著影响大肠杆菌O157:H7.7.的失活率.
- 收集后立即使用或最少存储床上用品可能会增强特定动物传染病原体的减少.
- 病床管理策略对于控制农业环境中动物性传染性细菌传播风险很重要.
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