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外源酸处理调节了草细胞悬浮培养中的蛋白质分泌

Dakalo Muthego¹, Sellwane J Moloi¹, Adrian P Brown²

¹Department of Plant Sciences, University of the Free State, Phuthaditjhaba, South Africa.

Plant signaling & behavior

|December 15, 2023

概括

酸 (ABA) 触发了的显著变化.

科学领域:

植物生物学植物生物学
生物化学生物化学
遗传学是一个遗传学.

背景情况:

干旱压力严重影响全球的作物产量.
酸 (ABA) 是一个关键的植物激素,调节干旱适应.
是一种重要的耐旱作物,使其应激反应机制变得非常重要.

研究的目的:

在模拟干旱条件下,研究如何对酸 (ABA) 产生反应.
为了确定参与干旱适应途径的特定蛋白质.
了解细胞外矩阵在ABA介导的应激反应中的作用.

主要方法:

使用了与外源性ABA处理的黑色生长的谷细胞悬浮培养.
用于相对和绝对量化 (iTRAQ) 的同位素标签来分析细胞内和分泌的蛋白质.
通过将体外试验结果与干旱压力植物的基因表达进行比较,验证了这些发现.

主要成果:

与细胞内蛋白质 (~7%) 相比,ABA的应用显著改变了分泌蛋白质的丰度 (~32%).
受 ABA 反应的分泌蛋白主要参与防御/解毒和细胞壁修饰.
编码这些ABA响应蛋白的基因在实际干旱压力下在植物中被激活.

结论:

细胞外基质在的ABA诱导的干旱适应中起着不成比例的重要作用.
糖果中的ABA信号激活了防御机制和细胞壁重塑.
这些由ABA介导的反应对于对干旱压力的弹性至关重要.

关键词:

阿巴阿巴阿巴是什么意思 Sorghum 的意思是 Sorghum 的意思细胞悬浮培养物细胞悬浮培养物干旱造成的压力是干旱.细胞外矩阵是细胞外矩阵.基因表达的基因表达方式这就是iTRAQQ.蛋白质分泌蛋白质分泌蛋白质分泌一些秘密一些秘密.总的可溶性蛋白质总量

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