来自Archaeal Pyrococcus furiosus的一种硫酶的晶体结构及其在的功能注释
Rashika Singh1, Vipin Kumar Mishra2, Amit Kumar Das1
1Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India.
Biochemical and biophysical research communications
|December 15, 2023
概括
梅瓦酸的途径.
科学领域:
- 生物化学 生物化学
- 结构生物学 结构生物学
- 古代生物生物学 古代生物生物学
背景情况:
- 异类是通过美酸盐路径合成的重要分子.
- 这种途径在真核生物和古生物中至关重要,用于生产必不可少的构建块,如异烯酸 (IPP) 和二甲基烯酸 (DMAPP).
研究的目的:
- 为了结构性地描述乙乙基CoA硫酶 (PFC_04095),这是来自古Pyrococcus furiosus的美瓦酸途径中的初始酶.
- 为了研究不同寻常的活性部位特征及其对酶活性的影响.
主要方法:
- 进行X射线晶体学以确定PFC_04095.5的2.7 Å分辨率结构.
- 接的PFC_04095-乙CoA复合物的分子动力学 (MD) 模拟.
主要成果:
- 晶体结构揭示了一个非典型的活性部位,缺少酸酶中常见的催化氨酸.
- 丁285 (HDAF动机) 似乎起到催化作用,但His335离Cys83太远,无法进行质子抽象,导致不活性.
- MD模拟表明在活性位点口袋中不稳定的乙-CoA结合.
结论:
- 结构和模拟数据表明,PFC_04095的稳定乙-CoA结合和酶活性取决于其与DUF35 (PFC_04100) 和HMGCS (PFC_04090) 蛋白质复合物的相互作用.
- 这突出了一个独特的机制来调节Pyrococcus furiosus中的美瓦酸途径.
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