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度和色度测量平台用于在水样中快速和灵敏地检测氧化

Kübra Doğan¹, Dilek Ünal Taş¹, Özgül Persil Çetinkol¹

¹Department of Chemistry, Middle East Technical University, 06800, Çankaya, Ankara, Turkey.

|December 15, 2023

概括

两种新的光学方法使用光和颜色变化快速检测氧化 (HCQ). 这些敏感平台为制药分析提供了实际的应用,即使是在尿液等复杂样品中.

科学领域:

分析化学分析化学
生物化学生物化学
材料科学材料科学材料科学

背景情况:

药品检测对于监测,制造和诊断至关重要.
由于使用量增加,COVID-19大流行增加了对氧化 (HCQ) 检测的需求.
现有的检测方法可能缺乏某些应用所需的速度,灵敏度或实用性.

研究的目的:

开发快速,灵敏和选择性的光学检测平台,用于氧化 (HCQ).
为了利用Thioflavin T (ThT) 和G-quadruplex DNA之间的相互作用,以及用于HCQ传感的银纳米粒子聚合.
验证这些方法在复杂的生物矩阵中的实际适用性.

主要方法:

度检测利用在HCQ和Tel24 G四重复DNA的存在下,提奥夫拉T (ThT) 的光灭.
基于酸盐覆盖银纳米粒子 (Cit-AgNPs) 的HCQ诱导聚合在盐水环境中的色度检测.
反应条件的优化和对类似小分子的选择性评估.

主要成果:

度方法显示线性范围为0.24-5.17μM,检测极限为120nM.
颜色测量方法表明线性范围为18.0-240.0 nM,检测极限为9.2 nM.
这两种方法都表现出良好的选择性,并且在增的人类尿样中实现了高平均恢复率 (75.4-110.2%和86.9-98.2%).

结论:

开发的度和色度平台提供了快速和灵敏的HCQ检测.
这些方法具有选择性,适用于复杂的矩阵,如人体尿液.
这些光学传感器为各种环境中的HCQ监控提供了有前途的方法.

关键词:

颜值测量颜色测量方法颜色测量方法检测检测检测检测检测度测量法度测量法氧化chloroquine 的使用情况.探测器探测器探测器

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