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对来自不同农场动物的牛奶中的α-氨酶抑制的分子对接研究

Priti Mudgil¹, Mouza Khamis Obaid Al Dhaheri¹, Maitha Saif Mohammed Alsubousi¹

¹Department of Food Science, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain, 15551, United Arab Emirates.

Journal of dairy science

|December 15, 2023

概括

发酵牛奶可以通过抑制胰腺α-氨基酶 (PAA) 来治疗糖尿病. 驼和山羊奶显示出最强的PAA抑制作用,这表明了新的治疗途径.

科学领域:

食品科学与技术食品科学与技术
生物化学生物化学
营养保健品营养保健品

背景情况:

乳制品因其健康益处而闻名,特别是在治疗糖尿病等生活方式障碍方面.
益生菌发酵是一种有效的生产具有治疗潜力的生物活性的方法.

研究的目的:

为了比较评估各种农场动物益生菌发酵牛奶中的的胰腺α-氨基酶 (PAA) 抑制活性.
识别具有显著PAA抑制性质的特定乳制品,并阐明它们的抑制机制.

主要方法:

通过使用益生菌Pediococcus pentosaceus MF000957.7.us发酵牛,,山羊和绵羊牛奶中的来抑制PAA的比较分析.
通过液体染色学四极飞行时间质谱法 (LC-QTOF-MS) 鉴定.
使用分子对接模拟来表征抑制机制.

主要成果:

发酵牛奶样本表现出PAA抑制活性,IC50值在2.39至36.1μg蛋白质相当量之间.
与其他益生菌相比,Pediococcus pentosaceus MF000957发酵牛奶在所有动物来源中都显示出优异的PAA抑制.
特定的,包括驼奶衍生的IMEQQQTEDEQQDK和山羊奶衍生的DQHQKAMKPWTQPK,被确定为强大的PAA抑制剂.

结论:

益生菌发酵有效地产生具有显著胰腺α-氨酸酶抑制潜力的牛奶.
驼和山羊奶通过PAA抑制显示出有前途的抗糖尿病特性.
发酵衍生的牛奶代表了开发功能性食品或糖尿病管理的营养品的可行策略.

关键词:

抗糖尿病生物活性.牛奶发酵牛奶发酵的过程分子对接的分子对接.益生菌是一种益生菌.抑制α-氨基酶的抑制.

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