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Echinococcus granulosus囊液通过促进巨细胞中的ROS抑制I型干扰素反应

Chunxue Fu¹, Chun Yang¹, Caiya Ni¹

¹NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, the First Affiliated Hospital/Shihezi University School of Medicine, Shihezi, Xinjiang, China; Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine, Shihezi, Xinjiang, China.

|December 15, 2023

概括

囊性赤球菌液 (EgCF) 通过增加活性氧物种 (ROS) 来降低巨细胞免疫力,从而阻断cGAS-STING-IRF3通路并降低I型干扰素反应. 通过NAC治疗恢复ROS水平会重新激活这种途径.

科学领域:

免疫学免疫学免疫学
寄生虫学的寄生虫学
细胞生物学细胞生物学

背景情况:

囊性乙球菌 (CE) 是一种寄生虫感染,其中乙球菌颗粒状囊性液体 (EgCF) 可能抑制巨细胞免疫力.
EgCF对关键的I型干扰素 (IFN-I) 反应的影响尚不清楚.

研究的目的:

调查EgCF是否影响巨细胞I型干扰素反应.
阐明潜在的分子机制,重点关注cGAS-STING-IRF3信号通路和活性氧物种 (ROS).

主要方法:

巨细胞用EgCF进行治疗,并用2'3'-cGAMP进行刺激.
测量了干扰素-β (IFN-β) 生产,cGAS-STING-IRF3信号激活,以及细胞内ROS水平.
评估了ROS抑制剂N-乙半氨酸 (NAC) 的作用.

主要成果:

EgCF显著降低了巨细胞中2'3'-cGAMP诱导的IFN-β的产生.
EgCF 抑制了 cGAS-STING-IRF3 信号通路.
EgCF治疗导致细胞内ROS水平增加.
NAC的使用恢复了cGAS-STING-IRF3信号和IFN-β的表达.

结论:

EgCF 损害了巨细胞介导的 I 型干扰素反应.
这种损伤是由EgCF诱导的ROS增加的媒介,这抑制了cGAS-STING-IRF3信号通路.

关键词:

埃奇诺科库斯颗粒性菌.巨细胞是一个巨细胞.这就是ROSOS ROS.这是一种I型干扰子.cGAS-STING 的使用情况.

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