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Updated: Jul 8, 2025

Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies

Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies

Published on: March 29, 2024

使用单细胞转录学和有机体对人类大脑血管细胞进行剖析.

Elizabeth E Crouch^1,2,3, Loukas N Diafos^4,5, Edward J Valenzuela^4,5

¹Department of Pediatrics, University of California San Francisco, San Francisco, CA, USA. elizabeth.crouch@ucsf.edu.

Nature protocols

|December 15, 2023

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此摘要是机器生成的。

研究人员开发了一种新方法来隔离和净化产前人类大脑血管细胞. 这种技术可以详细研究大脑发育和神经血管相互作用,使用像转录学和器官移植这样的技术.

科学领域:

神经科学是一个神经科学.
发展生物学发展生物学
血管生物学血管生物学

背景情况:

血管新生和神经新生对于大脑发育至关重要,但研究血管系统是具有挑战性的,因为细胞的微妙性和低丰度.
现有的方法限制了对脑血管细胞的全面分析.

研究的目的:

开发和验证一项用于净化产前人类大脑内皮细胞和壁画细胞的协议.
为了实现下游应用,如转录学,细胞培养和有机体移植.

主要方法:

流细胞计 (FACS) 用于净化产前人类大脑内皮细胞和壁细胞.
该协议被优化为24小时的死后间隔,以确保细胞健康和数据质量.
纯化的细胞被用于单细胞转录组学,体外培养试验和移植到皮质器官.

主要成果:

基于FACS的协议提供了从小组织样本中获得高产量的纯血管细胞.
纯化的细胞在培养中保持细胞类型标记物的表达超过3周.
移植到皮质器官中成功地重复了产前人类大脑的神经血管相互作用.

结论:

这种以血管为中心的方法为研究神经血管相互作用和人类大脑血管发育提供了一个高效和综合的平台.

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该协议为转录学和其他分子分析提供了高质量的数据生成.

该方法支持先进的体外和体内的脑血管发育模型.