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针对HIV-1 CD4诱导表位体的抗体的异位体与针对微生物的抗体共享

Shashwata Biswas¹, Takeo Kuwata¹, Soichiro Yamauchi²

¹Division of Clinical Retrovirology, Joint Research Center for Human Retrovirus Infection, Kumamoto University, Kumamoto, Japan.

|December 16, 2023

概括

针对HIV-1 CD4诱导表位体的抗体可能来自暴露于微生物蛋白质的B细胞. 这项研究确定了类似于这些抗体识别的微生物序列.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
微生物学微生物学

背景情况:

在HIV-1感染中,针对形状性CD4诱导 (CD4i) 表位的抗体 (Abs) 是常见的.
驱动抗CD4iAbs发展的精确机制在很大程度上是未知的.

研究的目的:

阐明HIV-1感染中抗CD4i Abs的起源和致病性抗原.
研究微生物抗原在抗CD4i Ab发展中的潜在作用.

主要方法:

利用抗异型 (aID) 单克隆抗体 (mAbs) 来从HIV-1感染个体中分离抗CD4i mAbs.
采用菌体显示器来分析抗艾滋病 mAb 结合的关键氨基酸序列.
来自微生物来源 (Caudoviricetes sp,Vibrio vulnificus) 的合成,用于反应性测试.

主要成果:

确定了抗CD4i Ab特征,包括IGHV1-69的使用和结合sCD4增强的HIV-1 Env.
发现了关键氨基酸序列和来自人类微生物群和传染病原体的蛋白质之间的相似之处.
微生物对抗艾滋病和一些抗CD4i mAbs.的反应性已被证明.

结论:

抗CD4i Abs在HIV-1感染中可能来自最初由微生物启动的B细胞反应.
微生物模仿可能是针对HIV-1 CD4i表位体的抗体开发的一个重要因素.

关键词:

艾滋病艾滋病艾滋病是什么?抗体是对抗体的重要组成部分.抗原/类/表位组的发生.

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