通过在废水中的细胞外化酶对抗微生物的水解
Natalie Wichmann1,2, Richard Gruseck1, Michael Zumstein1
1Division of Environmental Geosciences, Centre for Microbiology and Environmental Systems Science, University of Vienna, Josef-Holaubek-Platz 2, Vienna 1090, Austria.
Environmental science & technology
|December 16, 2023
概括
抗微生物 (AMP) 可能会被废水酶失活. 这项研究量化了酶活性和AMP生物转化,揭示了废水处理厂中保留的特定水解部位.
科学领域:
- 环境微生物学环境微生物学
- 生物化学 生物化学
- 污水处理 污水处理 污水处理
背景情况:
- 抗微生物 (AMP) 显示出作为新型抗生素的前景.
- 废水化酶可以使AMP无活化,减少它们向环境释放.
- 了解酶活性对于评估废水中的AMP命运至关重要.
研究的目的:
- 在废水处理过程中量化化酶活性.
- 评估废水酶在选定的AMP上的生物转化潜力.
- 研究废水酸酶的基质特异性.
主要方法:
- 从四个市政废水处理厂收集了细胞外提取物.
- 在不同治疗阶段的量化总酶活性.
- 通过废水酶评估AMP生物转化.
- 识别和量化AMP的水解产品.
主要成果:
- 在原始废水中检测到的酶活性最高.
- 观察到AMP对酶转化敏感性的显著变化.
- 水解发生在不同废水样本的特定,保存地点.
- 酶对AMP表现出相当大的基质特异性.
结论:
- 废水化酶表现出基质特异性,影响AMP命运.
- 在AMP上的水解点在不同的废水环境中得到保护.
- 这些发现有助于在废水中具有可预测的生物降解性的AMP的设计.
更多相关视频
05:46Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation
Published on: August 1, 2018
13.1K
10:13Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization
Published on: August 11, 2018
11.9K
相关概念视频
Antimicrobial Proteins
1000
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
1000
Surface Membrane Barriers
1.1K
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
1.1K
Lysosomal Hydrolases
3.8K
Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
3.8K
Protein Digestion
103.7K
Protein digestion begins in the stomach, where the highly acidic environment can easily disrupt protein structure by exposing the peptide bonds of polypeptide chains. After polypeptide chains are broken into individual amino acids by a series of digestive enzymes, the amino acids are transported to the liver via the bloodstream to produce energy.
103.7K
Hydrolysis
105.4K
Overview
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...
105.4K
