在播种阶段,OsMAPK6通过酸化和稳定OsICE1和OsIPA1来积极调节大米的耐寒性
Jiali Liu1, Jiaxin Liu2, Mingliang He2,3
1Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, 150040, China.
概括
OsMAPK6通过与OsICE1和OsIPA1.1相互作用来积极调节大米冷却耐受性. 这一发现为大米苗如何应对寒冷压力提供了新的见解.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 米对冷却温度非常敏感,严重影响作物产量.
- 在大米中冷却信号传导的精确分子机制尚未完全理解.
研究的目的:
- 为了研究OsMAPK6在大米冷却耐受性中的作用.
- 为了阐明涉及OsMAPK6在应对寒冷压力的信号通路.
主要方法:
- 分析OsMAPK6过度表达 (CAMAPK6-OE) 和突变 (dsg1) 线的冷却耐受性.
- 生理分析测量离子泄漏和细胞活力.
- 对冷却反应基因 (OsCBFs,OsTPP1) 的基因表达分析.
- 生物化学试验用于研究OsMAPK6酸化,相互作用以及对OsICE1/OsIPA1稳定性的影响.
主要成果:
- 与野生类型 (WT) 相比,dsg1突变显示了降低的冷却耐受性,而CAMAPK6-OE系则表现出增强的耐受性.
- 在寒冷压力下,dsg1表现出更严重的离子泄漏和细胞死亡.
- 对冷却反应基因的表达在dsg1中低于在WT和CAMAPK6-OE中.
- 冷却压力诱导了OsMAPK6的酸化,OsMAPK6与OsICE1和OsIPA1.1相互作用并稳定了它们.
结论:
- OsMAPK6在米冷却耐受性方面发挥着积极的调节作用.
- 涉及OsMAPK6,OsICE1和OsIPA1的冷诱导信号级联对于大米苗的冷反应至关重要.
相关概念视频
Responses to Heat and Cold Stress
13.5K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.5K
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
Regulation of Transpiration by Stomata
28.3K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
28.3K
MAPK Signaling Cascades
5.6K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.6K
Adaptations that Reduce Water Loss
25.6K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
25.6K
Responses to Drought and Flooding
10.7K
Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.
10.7K


