在微流体微息地模型中量化细菌和真菌培养的生长和营养消耗
Carlos Arellano-Caicedo1, Jason P Beech2, Martin Bengtsson3
1Center of Microbiology and Environmental System Sciences, University of Vienna, 1030 Vienna, Austria; Department of Biology, Lund University, 22362 Lund, Sweden.
STAR protocols
|December 16, 2023
概括
这项研究详细介绍了一种微流体协议,用于测量细菌生物质和细菌 (Pseudomonas putida) 和真菌 (Coprinopsis cinerea) 的营养分解. 该方法可以在受控的微观环境中对微生物活动进行定量分析.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 环境科学 环境科学
背景情况:
- 了解自然环境中的微生物行为需要研究它们的微环境.
- 微流体设备为微生物研究提供了受控的环境.
研究的目的:
- 提出一个详细的协议,用于量化细菌和真菌生物质和营养物质降解使用微流体.
- 使研究人员能够在精确控制的微观环境中研究微生物过程.
主要方法:
- 为微流体芯片制造开发协议,包括面具设计,主打印和聚甲基 (PDMS) 芯片制造.
- 用细菌 (Pseudomonas putida) 和真菌 (Coprinopsis cinerea) 培养的微流体芯片进行注射.
- 使用光显微镜,图像分析,绘图和统计方法量化微生物生物质和营养降解.
主要成果:
- 成功实施生物质和营养物质降解量化的微流体协议.
- 对细菌和真菌模型生物体的证明适用性.
- 为微流体微生物实验的详细图像分析和统计解释提供了一个框架.
结论:
- 提出的微流体协议提供了一种强大的方法,用于对微生物生物质和营养物质降解的定量分析.
- 这种技术有助于在定义的微环境中研究微生物生态生理学.
- 该协议可以适应各种微生物物种和微生物学和环境科学中的研究问题.
相关概念视频
01:23Microbial Growth Measurement: Direct Methods
Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
01:27Microbial Growth Measurement: Indirect Methods
Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...