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相关概念视频

RNA-seq03:21

RNA-seq

10.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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相关实验视频

Updated: Jul 8, 2025

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
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A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

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使用随机序列研究pri-miRNA处理的高通量协议.

Thi Nhu-Y Le1, Tuan Anh Nguyen1

  • 1Division of Life Science, The Hong Kong University of Science & Technology, Hong Kong, China.

STAR protocols
|December 16, 2023
PubMed
概括
此摘要是机器生成的。

我们开发了一种新的无放射性同位素的方法来研究微RNA (miRNA) 处理. 这种高通量协议有助于发现关键的RNA元素,并了解基因表达调节.

关键词:
基因表达 基因表达序列分析是指进行序列分析.

更多相关视频

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

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Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
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Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

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相关实验视频

Last Updated: Jul 8, 2025

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
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A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

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Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
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Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个
  • 生物化学 生物化学

背景情况:

  • 微处理器复合体对于微RNA (miRNA) 生物生成至关重要.
  • 它将初级miRNAs (pri-miRNAs) 处理成前体miRNAs,这是基因表达调节的关键步骤.

研究的目的:

  • 提出一种新的,高通量,无放射性同位素的协议,用于研究pri-miRNA处理.
  • 为了使primi-miRNA处理机制的探索,并确定关键的RNA元素.

主要方法:

  • 开发一个使用pri-miRNA基质随机序列的协议.
  • 基质制备,蛋白质净化和加工试验的详细步骤.
  • 用于分析处理结果的高通量测序的DNA库构建.

主要成果:

  • 提出的技术有助于研究primiRNA处理.
  • 它有助于发现影响处理效率的关键RNA元素.
  • 该方法阐明了通过miRNA生物生成调节基因表达的机制.

结论:

  • 这种无放射性同位素的协议为研究primiRNA处理提供了一个强大的工具.
  • 它促进了对miRNA生物发生及其在基因表达中的作用的理解.
  • 潜在的局限性包括数据分析的复杂性和需要专门设备.