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植物mRNA通过细胞外囊泡进入真菌病原体,以减少感染
Shumei Wang1, Baoye He1, Huaitong Wu1
1Department of Microbiology and Plant Pathology, Center for Plant Cell Biology, Institute for Integrative Genome Biology, University of California, Riverside, Riverside, CA, USA.
Cell host & microbe
|December 16, 2023
概括
植物在细胞外囊泡 (EV) 中向 Botrytis cinerea 发送信使RNA (mRNA),阻碍其感染. 这一发现提供了通过了解植物-真菌通信来对抗作物疾病的新策略.
科学领域:
- 植物病原体相互作用
- RNA生物学的RNA生物学
- 分子植物病理学 分子植物病理学
背景情况:
- 跨王国的RNA贩运,特别是小RNA,影响了宿主-微生物相互作用.
- 植物和真菌之间的其他RNA类型的转移,如信使RNA (mRNA),仍然在很大程度上未被探索.
研究的目的:
- 研究宿主植物mRNAs在真菌病原体中的潜在转移.
- 确定这些转移的mRNA是否具有功能并影响感染过程.
主要方法:
- 采用光RNA吸收体记者系统 (Broccoli) 来可视化细胞外囊泡 (EVs) 和真菌细胞中的宿主mRNA.
- 使用翻译核糖体亲和力净化 (TRAP) 分析和多体分析来评估受体真菌细胞中的mRNA翻译.
- 在Botrytis cinerea中进行了子宫外表达研究,并分析了Arabidopsis淘汰突变物.
主要成果:
- 证明了Arabidopsis thaliana通过EVs向真菌病原体Botrytis cinerea提供mRNA.
- 证实这些转移的宿主mRNA在真菌细胞内被翻译.
- 表明B. cinerea蛋白质中转移的mRNAs的异位表达对感染有害,相应的Arabidopsis突变更容易受到感染.
结论:
- 植物拥有通过将功能mRNA运输到致病菌中来对抗真菌感染的机制.
- 这种mRNA转移破坏了真菌感染,突出了一个新的植物防御策略.
- 结果提供了对跨王国RNA通信的见解,并为开发作物抗病能力提供了潜在的途径.
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