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CTRP6促进巨细胞的炎症反应,其缺乏会减轻LPS诱导的炎症

Cheng Xu¹, Dylan C Sarver¹, Xia Lei²

¹Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

The Journal of biological chemistry

|December 16, 2023

概括

蛋白CTRP6 (C1q/TNF相关蛋白6) 激活了巨细胞中的炎症基因程序和信号通路,促进了与肥胖相关的炎症. 失去CTRP6会抑制炎症反应,这表明它在调节组织炎症方面发挥了作用.

科学领域:

免疫学免疫学免疫学
代谢性疾病是一种代谢性疾病.

背景情况:

巨细胞是炎症和组织平衡的关键调节者.
CTRP6 (C1q/TNF相关蛋白6号) 与肥胖相关的炎症有关.
目前尚不完全了解CTRP6影响巨细胞功能的精确机制.

研究的目的:

阐明巨细胞中由CTRP6调节的基因程序和信号通路.
研究CTRP6在巨细胞炎症反应和代谢转变中的作用.

主要方法:

对小鼠骨髓衍生的巨细胞 (BMDMs) 的转录和蛋白分析.
药理上抑制MAPK和NF-κB信号通路.
评估巨细胞对LPS刺激的反应.
来自CTRP6缺乏小鼠的BMDMs的分析.
用小鼠进行体内研究,以评估LPS诱导的炎症和低温.

主要成果:

在BMDM中,CTRP6治疗上调了促炎和抑制了抗炎基因表达.
CTRP6激活了p44/42-MAPK,p38-MAPK和NF-κB信号,增强了细胞因子的分泌.
CTRP6促进了有氧糖解,降低了氧化代谢,并增加了线粒体ROS的产生.
来自CTRP6缺乏的小鼠的BMDM显示出基线炎症减少和抑制LPS诱导的反应.
在小鼠中失去CTRP6减轻了LPS诱导的炎症和低温.

结论:

CTRP6激活炎症通路,并启动巨细胞,以增加对炎症刺激的反应.
CTRP6影响巨细胞的新陈代谢,使其转向一种促炎性表型.
在体外和体内,CTRP6在调节巨细胞介导炎症方面发挥着重要作用.

关键词:

在CTRP中,它是最重要的.在LPS中使用LPS.这是一种炎症炎症炎症炎症.巨细胞是一个巨细胞.光蛋白质组学光蛋白质组学光蛋白质组学信号传输信号传输翻译学翻译学翻译学翻译学

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