相关实验视频

Updated: Aug 4, 2026

A Proinflammatory, Degenerative Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease.

A Proinflammatory, Degenerative Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease.

Published on: February 14, 2021

生物信息学分析和识别与脊椎间盘退化相关的铁亡相关的枢纽基因

Feng Jiang¹, Xinxin Li¹, Zhiyang Xie²

¹Southeast University Medical College, No. 87, Dingjiaqiao Road, Nanjing, 210009, Jiangsu, China.

Biochemical genetics

|December 16, 2023

概括

细胞死亡过程 - - 铁亡与由于椎间盘退化 (IDD) 的腰部疼痛 (LBP) 有关. 这项研究确定了关键的铁灭基因 (PTEN,EGFR) 作为IDD的潜在生物标志物.

科学领域:

生物医学研究的研究.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

腰部疼痛 (LBP) 影响大约80%的人,通常与椎间盘退化 (IDD) 有关.
铁,一种由铁和脂质过氧化驱动的编程细胞死亡形式,在疾病发病过程中越来越被认可,但其在IDD中的作用尚不清楚.

研究的目的:

确定关键的铁与相关的基因和途径,涉及到IDD的发展和进展.
为了探索与亡相比,IDD中的铁亡的特异性.

主要方法:

分析了三个GEO微阵列数据集用于IDD.
利用权重基因共同表达网络分析 (WGCNA) 来识别与IDD相关的基因模块.
叠加的IDD模块与ferroptosis和apoptosis基因组用于识别相关的基因.
功能丰富,蛋白质-蛋白质相互作用网络分析和物流回归被用来识别枢纽基因.

主要成果:

确定了35个与铁灭相关的模块基因 (FRMG) 和142个与灭相关的模块基因 (ARMG).
确定PTEN和EGFR是显著的铁灭枢纽基因,CTNNB1是灭枢纽基因,区分IDD患者和对照组 (P<0.05).
在IDD中,ferroptosis表现出比apoptosis更高的特异性,ROC AUC分别为0.792和0.730.

结论:

铁死在IDD的发病和进展中起着重要作用.
PTEN和EGFR是IDD的潜在诊断生物标志物.
这些发现为IDD中的铁亡机制提供了新的见解,并提出了潜在的治疗点.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.铁化是铁化的一种.椎间盘退化椎间盘退化在WGCNA中,WGCNA是WGCNA.

更多相关视频

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration

Published on: July 8, 2021

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics

Published on: March 15, 2024

相关概念视频

Herniated Intervertebral Disc l: Introduction

Herniated Intervertebral Disc l: Introduction

Intervertebral disc herniation refers to the displacement of the nucleus pulposus (the gel-like inner core of the disc) through a tear or weakened area in the annulus fibrosus (the outer fibrous ring). The displaced disc material extends beyond the normal boundaries of the disc space and may compress or irritate nearby spinal nerve roots or, less commonly, the spinal cord.Etiology and Risk FactorsHerniation commonly results from degeneration, in which aging reduces disc hydration and...

Degenerative Disc Disease I: Introduction

Degenerative Disc Disease I: Introduction

Degenerative disc disease is a chronic condition in which intervertebral discs gradually lose structure and function. It is not infectious or autoimmune; rather, it results from age-related biochemical and mechanical changes, influenced by genetic, metabolic, and environmental factors.Structure and Function of DiscsThe spine contains 23 intervertebral discs that absorb load, distribute forces, maintain spacing, and allow flexibility. Each disc consists of a nucleus pulposus, a gel-like core...

Degenerative Disc Disease ll: Pathophysiology

Degenerative Disc Disease ll: Pathophysiology

The symptoms of degenerative disc disease arise from a combination of mechanical compression, vascular compromise, and biochemical inflammation, which together disrupt nerve function and produce pain.Mechanical CompressionDisc degeneration reduces height and elasticity, predisposing to herniation of the nucleus pulposus, a major cause of radicular pain. Herniations may be protrusion (bulging with intact annulus), extrusion (nucleus extends beyond disc but remains connected), or sequestration...