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Updated: Jul 8, 2025

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
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来自Saccharothrix sp.的高效N-氧化酶的表征 和其在阿佐米辛合成中的应用

Chuanle Fan1,2,3, Fang Zhou1,2,3, Wei Huang1,2,3

  • 1CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China.

Biotechnology for biofuels and bioproducts
|December 16, 2023
PubMed
概括

一种新型酶SaRohS有效地将2-aminoimidazole转化为azomycin,这是一个有价值的抗菌前体. 局部定向突变显著增强了其催化活性,为阿佐米辛生产提供了一个有前途的生物催化途径.

关键词:
类似于血氧酶的二氧酶.亚化合物是一种化合物.亚胺醇是一种胺醇.位点定向突变是指向该位点的突变.全细胞生物催化剂的整细胞生物催化剂

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Direct alkylation of ammonia produces polyalkylated amines, along with a quaternary ammonium salt. To exclusively prepare primary amines, the azide synthesis method can be used.
Azide ions act as good nucleophiles and react with unhindered alkyl halides to form alkyl azides. Alkyl azides do not participate in further nucleophilic substitution reactions, thereby eliminating the chances of polyalkylated products. Alkyl azides are reduced by hydride-based reducing agents, like lithium aluminum...
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