在甲基应激下,海洋 rotifer Brachionus plicatilis 的转录组规则的变化
Hangyu Lin1, Yanlin Wei1, Songzhang Li2
1College of Fisheries, Southwest University, Chongqing 400715, China; Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, 400715, China.
Comparative biochemistry and physiology. Part D, Genomics & proteomics
|December 17, 2023
概括
甲基 (MeHg) 暴露会影响鸟,影响谷甲代谢和溶酶体通路. 转录组分析揭示了水生食物链中明显的排毒和毒性机制.
科学领域:
- 环境毒理学环境毒理学
- 水生生态学 水生生态学
- 分子生物学分子生物学
背景情况:
- (Hg) 是一种全球性污染物,在水生环境中转化为有毒的甲基 (MeHg).
- MeHg在食物链中生物累积,对人类健康构成风险.
- 动物浮游生物,如 rotifer Brachionus plicatilis,是水生生态系统和食物网的关键.
研究的目的:
- 为了研究MeHg对B. plicatilis. rotifer的影响.
- 为了阐明基底的分子机制 MeHg毒性在罗提弗.
- 为了比较对直接MeHg暴露和饮食MeHg暴露的反应.
主要方法:
- 通过直接浸泡,B. plicatilis暴露于MeHg.
- 通过食用受污染的藻类 (Chlorella pyrenoidesa) 暴露于B. plicatilis的MeHg.
- 转录组分析以确定受影响的生物途径.
主要成果:
- 直接暴露于MeHg上调了谷氨代谢途径的排毒.
- 饮食中的MeHg暴露丰富了溶酶体和隙信号通路,表明自功能障碍和神经系统障碍.
- 在两种暴露途径中,细胞染色体P450通路都被显著丰富,这表明它在MeHg耐药性中发挥了作用.
结论:
- 根据暴露途径,MeHg对虫产生不同的分子效应.
- lysosome 和 notch 信号通路与 MeHg 诱导的细胞和神经效应有关.
- 需要进一步的研究来了解水生食物链中MeHg的毒性.
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