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2-脱氧葡萄糖改善了由于暴露于腹膜透析液体而发生的腹膜介质和内皮膜屏障功能干扰

Eleanna Pitaraki¹, Rajesh M Jagirdar¹, Erasmia Rouka²

¹Department of Physiology, Faculty of Medicine, School of Health Sciences, University of Thessaly, BIOPOLIS, 41500, Larissa, Greece.

Biochemical and biophysical research communications

|December 17, 2023

概括

在腹透析液 (PDF) 中添加二氧化葡萄糖 (2-DG) 可减轻纤维化,并改善腹膜 (PM) 的中皮质和内皮质屏障功能. 这项研究证实了2-DG的存在.

科学领域:

腹膜透析研究腹膜透析研究
细胞生物学细胞生物学
膜运输是通过膜运输来实现的.

背景情况:

腹膜透析 (PD) 和液体 (PDF) 可以导致腹膜膜 (PM) 纤维化和高血管性,导致PM退化.
2-脱氧葡萄糖 (2-DG) 通过抑制半体转化为半体转化 (MMT) 来显示出作为抗纤维素剂的潜力.

研究的目的:

研究2-DG与各种PDF相结合对PM中介质和内皮质屏障透性的影响.
为了证实2-DG对腹膜细胞的抗纤维作用.

主要方法:

使用的凝收缩测定与中皮细胞 (MeT-5A) 和内皮细胞 (EA.hy926) 培养在不同的PDF与/或没有2-DG.
对αSMA和CDH2基因表达进行了qPCR.
通过使用跨膜电阻 (R_TM) 和FITC-dextran扩散来评估屏障透性.
分析了CLDN-1至-5,ZO1,SGLT1和SGLT2基因的mRNA表达.

主要成果:

2-DG降低了细胞收缩性,并改变了中细胞中的αSMA和CDH2基因表达.
在内皮细胞中观察到αSMA和CDH2的类似变化,在LPDF/2-DG下αSMA下降.
2-DG减轻了PDF诱导的屏障功能变化,得到了改善的R_TM,减少了右运输,并使紧结和传送基因的表达正常化.

结论:

用2-DG补充PDF可以减少MMT.
2-DG 改善了PM 中间皮质和内皮质屏障的功能透性特性.
2-DG是一个有希望的策略来抵消PD诱导的腹膜损伤.

关键词:

2 - 脱氧葡萄糖是二氧化葡萄糖.克劳迪恩 (Claudin) 是一个很好的球员.介质质地质层 (Mesothelium) 是一个层.类似细胞的透性腹膜透析是指腹膜透析.紧密的结口是紧密的结口.

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