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通过动脉病毒非结构蛋白1抑制TRIM19,促进病毒复制

Chia-Ming Su¹, Yu Fan Hung¹, Junyu Tang¹

¹Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 South Lincoln Ave, Urbana, IL 61802, United States.

|December 17, 2023

概括

含有三方基因 (TRIM) 的蛋白质,比如Promyelocytic白血病 (PML) 蛋白质,对于抗病毒反应至关重要. 动脉病毒,包括PRRSV,抑制PML核体 (NBs) 作为一种保存的免疫逃避策略.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

含有三方基因 (TRIM) 的蛋白调节抗病毒细胞因子诱导和病毒复制.
亲细胞白血病 (PML) 蛋白 (TRIM19) 组织PML核体 (NBs),对细胞平衡和抗病毒防御至关重要.
猪生殖和呼吸系统综合征病毒 (PRRSV) 通过其非结构蛋白1 (nsp1) 抑制I型干扰素 (IFN) 反应.

研究的目的:

调查PML核体 (NBs) 在PRRSV感染中的作用.
为了确定PRRSV是否操纵PMLNBs以逃避免疫.
探索这种操纵是否在其他动脉病毒中得到保护.

主要方法:

在PRRSV感染期间量化PMLNBs.
在PML异型过度表达时评估PRRSV复制.
在PML基因沉默后评估PRRSV复制.
在其他动脉病毒中检查nsp1的PMLNB抑制.

主要成果:

每个核的PML NBs被显著降低了PRRSV感染.
PML过度表达抑制了PRRSV复制,而PML沉默增强了它.
PRRSV nsp1抑制了PML NBs,这种现象在其他动脉病毒 (EAV,LDV,SHFV) 中观察到.

结论:

在限制PRRSV复制方面,PML NBs发挥着重要作用.
动脉病毒,包括PRRSV,采用一种保守的策略,通过nsp1抑制PMLNBs,以逃避天生的免疫力.

关键词:

IFN的对抗性 IFN的对抗性免疫逃避是免疫逃避的一种方式.这是PML的PML.PRRSVV 在这个问题上.促细胞白血病蛋白质是什么在 TRIM19 中,这是一个nsp1sp1nsp1

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