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循环ZNF609通过miR-145-5p/KLF4轴及其翻译功能调节肺纤维化
Wenqing Sun1, Siyun Zhou1, Lan Peng1
1Department of Occupational Medical and Environmental Health, Key Laboratory of Modern Toxicology of Ministry of Education, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, 211166, China.
Cellular & molecular biology letters
|December 17, 2023
概括
循环RNA ZNF609 (circZNF609) 在二氧化诱导的肺纤维化中是下调的. 抑制circZNF609抑制纤维细胞激活和肺纤维化,提供了一个潜在的新治疗标.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 肺部医学 肺部医学
背景情况:
- 肺纤维化是一种渐进的肺病,治疗选择有限.
- 由于二氧化引起的肺纤维化病原体尚未完全理解.
- 循环RNAs (circRNAs) 与疾病有关,但它们在引起的肺纤维化中的作用尚不清楚.
研究的目的:
- 为了研究circZNF609在二氧化引起的肺纤维化中的作用.
- 为了阐明circZNF609在纤维细胞激活中的分子机制.
主要方法:
- 定量实时聚合酶链反应 (qRT-PCR) 用于测量基因表达.
- RNA免疫沉降 (RIP) 和m6ARNA免疫沉降 (MeRIP) 的测试.
- 用纤维细胞细胞系进行体外研究和用气诱导的小鼠模型进行体内研究.
主要成果:
- 在激活的纤维细胞和纤维化肺组织中,circZNF609的下调.
- 在小鼠中,circZNF609过度表达抑制了纤维细胞激活和二氧化诱导的肺纤维化.
- circZNF609通过miR-145-5p/KLF4轴和circZNF609编码的调节肺纤维化,其表达因N6-甲基氨酸 (m6A) 修饰而调节.
结论:
- circZNF609是纤维细胞激活和二氧化诱导的肺纤维化的一个关键调节器.
- circZNF609及其衍生可以作为肺纤维化治疗的新治疗标.
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