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通过STAT3/VEGF信号传递,REC8调节神经母细胞细胞的增殖,迁移,入侵和血管生成

Qiang Wang¹, Wei Fan², ZengHui Hao²

¹Department of Pediatric Surgery, The Sixth Affiliated Hospital of Harbin Medical University, Harbin, 150001, China. doctor_wang@vip.163.com.

Journal of the Egyptian National Cancer Institute

|December 17, 2023

概括

准REC8,一个介质凝聚复合体中的蛋白质,显著抑制神经母细胞瘤的生长,迁移和血管生成. 击败REC8诱导细胞循环停止和细胞亡,为高风险神经母细胞瘤提供了一个有希望的治疗策略.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

神经母细胞瘤是一种普遍存在的儿童癌症,高风险病例的预后不佳.
介质凝聚复合体的组成部分REC8与各种恶性瘤有关.
确定新的治疗点对于改善神经母细胞瘤治疗结果至关重要.

研究的目的:

调查REC8淘汰对神经母细胞瘤细胞系 (SH-SY5Y和SK-N-AS) 的影响.
阐明REC8在神经母细胞瘤中的作用背后的分子机制.
评估REC8作为神经母细胞瘤的潜在治疗点.

主要方法:

小干扰 (si) RNA技术被用来击败REC8.8.
定量逆转录酶聚合酶连锁反应 (qRT-PCR) 和西部涂抹验证了REC8下调.
细胞计数工具-8 (CCK-8),流细胞计量,管形成试验和Transwell测试评估了细胞增殖,细胞循环,细胞亡,血管生成,迁移和入侵.

主要成果:

通过诱导G1阶段细胞循环停止,REC8下调显著减少神经母细胞细胞的增殖.
Knockdown 的 REC8 抑制了细胞迁移,入侵和血管生成.
抑制REC8表达减少了神经母细胞瘤细胞中STAT3/VEGF信号通路的激活.
在神经母细胞瘤细胞中,REC8敲除增强了亡.

结论:

准REC8是神经母细胞瘤的潜在治疗策略.
对REC8的下调抑制了神经母细胞瘤中关键的致癌过程.
在STAT3/VEGF途径中REC8的作用表明了下游的治疗目标.

关键词:

血管新生的产生.这是入侵.神经母细胞瘤是一种神经母细胞瘤.在 REC8 中使用.在STAT3/VEGF中.

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