使用Paenibacillus的微生物细胞工厂:现状和前景
Panhong Yuan1, Ziyan Chen1, Mengtao Xu1
1College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, Zhejiang, China.
Critical reviews in biotechnology
|December 18, 2023
概括
被认为是安全的Paenibacillus细菌提供了农业和工业的好处. 基因工具的进步正在释放它们作为生物制造的微生物底盘的潜力,扩大在酶,生物修复和抗微生物药物中的应用.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 合成生物学 合成生物学
背景情况:
- 帕尼巴西勒斯是一种普遍公认的安全 (GRAS) 细菌,在农业,医学,工业和环境修复方面具有多样化的应用.
- 这些根系细菌促进植物生长,降解毒素,并产生有价值的酶和抗菌.
- 在历史上,有限的基因操纵工具限制了Paenibacillus生物资源的充分利用.
研究的目的:
- 审查Paenibacillus生物制品的应用程序.
- 讨论最近对Paenibacillus.遗传操纵系统的进展.
- 突出未来的挑战和Paenibacillus作为生物制造的微生物底盘的潜力.
主要方法:
- 审查关于Paenibacillus应用和遗传工具的现有文献.
- 分析基因操纵技术的进步,包括穿等离子体,促进剂和CRISPR.
- 检查开发的基因转化系统,如青素介导转化,电穿孔和介导转化.
主要成果:
- 在开发Paenibacillus.遗传操纵工具和转化系统方面取得了重大进展.
- 这些进步使Paenibacillus能够被用作生物制造的微生物底盘.
- 扩展的应用包括工业酶,生物修复,生物吸附,表面活性剂和抗菌剂.
结论:
- 帕尼巴西勒斯 (Paenibacillus) 具有巨大的潜力,可以作为采矿生物资源的微生物底盘.
- 持续开发基因操纵系统对于实现这一潜力至关重要.
- 这种属性为可持续的生物制造和各种工业应用提供了有前途的途径.
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