TML1 和TML2 协同调节节节,并影响MEDICAGO TRUNCATULA 中的 ARBUSCULAR MYCORRHIZA
bioRxiv : the preprint server for biology
|December 18, 2023
概括
在Medicago truncatula中MtTML1和MtTML2基因的突变显著增加结节的形成,双重突变显示出协同作用的20倍增加. 这些发现提供了关于植物微生物共生调节的见解.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物与微生物的相互作用
- 遗传学 是一个遗传学.
背景情况:
- 植物的共生过程,如结节 (固定) 和状菌根 (吸收),对于营养的获取至关重要.
- 结节自主调节 (AON) 和菌根自主调节 (AOM) 是控制这些共生的负反机制,共享共同的调节组件.
- 太多的爱 (TML) 蛋白质家族参与调节结节,同类物MtTML1和MtTML2在Medicago truncatula中被发现.
研究的目的:
- 研究MtTML1和MtTML2在Medicago truncatula共生中的作用.
- 在MtTML1和MtTML2中生成和描述突变,以了解它们在结节和状菌根中的功能.
- 探索突变在MtTML1和MtTML2中的潜在协同效应.
主要方法:
- 使用CRISPR-Cas9的向突变发生,在MtTML1和MtTML2中产生稳定的突变.
- 选了一个转子子突变性库,以确定额外的等位基变异.
- 对单个和双重突变的表型分析进行,以评估结节数量和状菌根殖民.
主要成果:
- 与野生类型植物相比,MtTML1或MtTML2中的单个突变体在结节形成中增加了2-3倍.
- 双重突变 (MtTML1/MtTML2) 显示出协同效应,形成的结节大约是野生类型的20倍.
- 在双重突变中观察到状菌根殖民的轻微增加,这表明在AM共生调节中起的作用很小.
结论:
- MtTML1和MtTML2在Medicago truncatula中对结节的负调节中起着重要的作用,并且可能具有协同作用.
- 遗传补偿机制可能有助于在双重突变中观察到的协同效应.
- 产生的突变物是进一步剖析植物微生物共生背后的分子机制的宝贵工具.
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