在发芽酵母中,Gtr1/2和Pib2依赖TORC1调节的分子逻辑
Jacob H Cecil1, Cristina M Padilla1, Austin A Lipinski2
1Department of Molecular and Cellular Biology, University of Arizona, Tucson, AZ, 85721.
bioRxiv : the preprint server for biology
|December 18, 2023
概括
拉巴氨酸激酶复合物I (TORC1) 途径的目标使用Gtr1/2和Pib2来控制细胞生长. 这种双重系统驱动了三个不同的TORC1信号状态,从快速增长到静止.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 拉帕氨酸激酶复合物I (TORC1) 的目标是细胞生长和细胞代谢的关键调节者.
- 在Saccharomyces cerevisiae中,营养物质激活TORC1涉及Gtr1/2 GTPases和Pib2蛋白,但它们的合作机制尚不清楚.
研究的目的:
- 阐明Gtr1/2和Pib2在调节TORC1信号中的合作作用.
- 为了定义TORC1的独特信号状态,以响应营养的可用性.
主要方法:
- 研究了酵母中的Gtr1/2和Pib2之间的相互作用.
- 在不同的营养条件下监测TORC1信号状态.
主要成果:
- 确定了由Gtr1/2和Pib2.2控制的三个不同的TORC1信号状态.
- 状态1 (Gtr1/2开启,Pib2开启):在富含营养的条件下快速生长.
- 状态2 (Gtr1/2关闭,Pib2打开):适应性/缓慢的生长在贫穷的介质中.
- 状态3 (Gtr1/2关闭,Pib2关闭):在饥饿下静止.
结论:
- Gtr1/2和Pib2系统为TORC1.1提供了一个多州监管机制.
- 这种双调节模型解释了单个酶如何能够驱动细胞对环境线索的多样性反应.
- 表明类似的多层次监管策略可能存在于其他信号通路中.
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