皮质N-甲基-D-酸盐 (NMDA) 受体通过影响脏自我调节来调解脏血管扩张
Cesar A Romero1, Jasmine Lim1, Hong Wang2
1Renal Division, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia, USA.
bioRxiv : the preprint server for biology
|December 18, 2023
概括
上皮细胞中的N-甲基-D-酸盐受体 (NMDAR) 促进 afferent动脉血管扩张. 这一过程涉及通过管状球体反激活ENaC通道,特别是在雌性小鼠中.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學專業.
- 分子生物学分子生物学
- 生理学 生理学 生理学
背景情况:
- N-甲基-D-酸盐受体 (NMDARs) 对于大脑功能至关重要,但它们在生理学中的作用在很大程度上是未知的.
- NMDAR 抑制剂会影响血清和淋巴细胞过率 (GFR),这表明功能.
- 我们研究了NMDARs在远端脏在通过连接管管-球膜反 (CNTGF) 和ENaC激活来调节 afferent arteriole血管扩张中的作用.
研究的目的:
- 为了研究NMDARs在脏中的表达和功能.
- 确定远端脏中的NMDAR是否通过CNTGF调节脏血液流动.
- 阐明NMDARs和ENaC在调解血管扩张中的相互作用.
主要方法:
- 对管体特异性转录组数据库的分析.
- 分子生物学和显微镜技术检测细胞中的NMDAR亚单元表达.
- 在小鼠脏中进行NMDAR激动剂和阻断剂的体外微 perfusion.
- 在体内进行脏微穿孔,以测量停止流量压力.
- 使用mpkCCD细胞和分裂开放管道的电生理学研究来评估ENaC活动.
主要成果:
- NMDAR子单元表达在脏沿线,特别是ENaC阳性细胞中,在雌性小鼠中表达的数量更高.
- 连接管中的NMDAR激活诱导了依赖CNTGF和ENaC活动的 afferent-arteriole血管扩张.
- 在体内研究证实,在NMDAR激活后,CNTGF相关的血管扩张.
- 激活NMDAR会增加氨酸敏感电流和细胞中的ENaC活性.
结论:
- 表皮NMDARs存在于脏中,包括表达ENaC的细胞,表达的性别差异.
- 皮质NMDAR通过CNTGF通过增强ENaC活性来调解血管扩张.
相关概念视频
01:28Glomerular Filtration Rate and its Regulation
2.8K
The Glomerular Filtration Rate (GFR) is a measure of kidney function, reflecting the volume of filtrate formed per minute in the kidneys. On average, GFR is approximately 125 mL/min in males and 105 mL/min in females. Maintaining a relatively constant GFR is essential for the kidneys to effectively regulate body fluid homeostasis and maintain extracellular stability.
GFR regulation involves two primary intrinsic controls: the myogenic and tubuloglomerular feedback mechanisms.
The myogenic...
GFR regulation involves two primary intrinsic controls: the myogenic and tubuloglomerular feedback mechanisms.
The myogenic...
2.8K
01:10Nephrons
2.5K
The kidneys are intricate organs with millions of working units known as nephrons. Each nephron features two major structures: the renal corpuscle, which facilitates blood plasma filtration, and the renal tubule, which handles the glomerular filtrate. Blood supply is directly linked to the nephrons. The renal corpuscle consists of the glomerulus, a capillary network, and the Bowman's capsule, a double-walled epithelial structure that encases the glomerulus. The filtering of blood plasma...
2.5K
01:17Autoregulation of Blood Flow
2.3K
Autoregulation mechanisms are characterized by their inherent capacity for self-regulation without necessitating specific nervous stimulation or endocrine control. These mechanisms facilitate the adjustment of blood flow and, therefore, perfusion specific to each tissue region. This self-regulation encompasses chemical signals and myogenic controls.
Chemical Signaling in Autoregulation
Chemical signaling operates at the precapillary sphincter level, inciting either contraction or relaxation....
Chemical Signaling in Autoregulation
Chemical signaling operates at the precapillary sphincter level, inciting either contraction or relaxation....
2.3K
01:33Hormonal Regulation
33.2K
The renin-aldosterone system is an endocrine system which guides the renal absorption of water and electrolytes, thus managing blood pressure and osmoregulation. Activation of the system begins in the kidneys with a small cluster of cells adjacent to the afferent and efferent blood vessels of the renal corpuscle. As the nephrons are filtering blood, juxtaglomerular cells monitor blood pressure. If they detect a decrease in pressure, they release the hormone renin into the bloodstream.
33.2K
01:18Hypertension and Regulation of Blood Pressure
2.1K
Hypertension, the most common cardiovascular disease, is diagnosed through repeated measurements of elevated blood pressure. Its risks, including damage to the kidney, heart, and brain, are directly proportional to blood pressure levels. Starting from 115/75 mm Hg, the risk of cardiovascular disease doubles with each increment of 20/10 mm Hg. The diagnosis relies on blood pressure measurements, not on patient symptoms, as hypertension is often asymptomatic until end-organ damage is imminent or...
2.1K
01:13Introduction to Urinary System
2.7K
The urinary system consists of two kidneys, two ureters, the urinary bladder, and the urethra.
The kidneys are bean-shaped organs located in the retroperitoneal space, on either side of the vertebral column, between the T12 and L3 vertebrae. They are partially protected by the rib cage and surrounded by perirenal fat, which provides cushioning. They are responsible for urine formation and play critical roles in regulating blood pressure, electrolyte levels, and hormone production. The ureters...
The kidneys are bean-shaped organs located in the retroperitoneal space, on either side of the vertebral column, between the T12 and L3 vertebrae. They are partially protected by the rib cage and surrounded by perirenal fat, which provides cushioning. They are responsible for urine formation and play critical roles in regulating blood pressure, electrolyte levels, and hormone production. The ureters...
2.7K