血液成分中Kynurenine途径代谢物的稳定性研究 定义最佳的血液处理条件
Benjamin Heng1, Ananda Staats Pires1, Sharron Chow1
1Macquarie Medical School, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, NSW, Australia.
International journal of tryptophan research : IJTR
|December 18, 2023
概括
基努瑞宁通路 (KP) 代谢物稳定性因血液基因和处理时间而异. 血清或血是首选的,收集后的立即处理确保了疾病研究的最佳分析数据.
科学领域:
- 生物化学 生化学
- 代谢学 代谢学 代谢学
- 临床化学 临床化学
背景情况:
- kynurenine 途径 (KP) 对 (TRP) 代谢和细胞能量至关重要.
- KP失调与各种人类疾病有关,其代谢物可能作为生物标志物或治疗点.
- 准确测量KP代谢物对于临床病理相关性至关重要,但其在血液样本中的稳定性尚不清楚.
研究的目的:
- 评估全血,血和血清中的KP代谢物度.
- 评估预分析样本处理时间对不同血基基因组的KP代谢物稳定性的影响.
主要方法:
- 在匹配的全血,血和血清样本中比较KP代谢物度.
- 在采集血液的延迟处理 (24小时在4°C) 后对代谢物稳定性的分析.
主要成果:
- 血清和血显示了比全血更高的KP代谢物度.
- 在加工前在4°C储存24小时后,KP代谢物度显著下降.
- 血基类型和处理延迟都会影响KP代谢物的稳定性.
结论:
- 血清或血是KP代谢物分析的首选血液基准.
- 建议在采集完毕后立即从全血中分离血清或血,以获得最佳和稳定的分析KP数据.
- 了解这些预分析因素对于可靠的生物标志物发现和在KP相关疾病中确定治疗点至关重要.
更多相关视频
07:04A High-performance Liquid Chromatography Measurement of Kynurenine and Kynurenic Acid: Relating Biochemistry to Cognition and Sleep in Rats
Published on: August 19, 2018
10.6K
04:17Author Spotlight: Advancing Cellular and Protein Engineering to Control Biological Functions and Develop Novel Therapies
Published on: September 27, 2024
704
相关概念视频
01:20Blood Studies for Cardiovascular System I: Cardiac Biomarkers
161
Cardiac biomarkers are enzymes, proteins, and hormones released into the blood when cardiac cells are injured. They are powerful tools for triaging.
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...
161
01:19Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers
95
Cardiac biomarkers are critical in diagnosing, prognosing, and managing cardiovascular diseases. Routine measurement of specific biomarkers such as B-type natriuretic peptide (BNP), C-reactive protein (CRP), and homocysteine (Hcy) is common practice in clinical settings to evaluate heart function and predict cardiovascular events.
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
95