在小麦的miR172目标部位AP2内发生的突变同质物调节了小体大小和拉基斯内部节点长度
Agetha Bigie Nanape1, Hlaing Moe Haine1, Kazuhiko Sugimoto2
1Graduate School of Bioresources, Mie University, 1577 Kurimamachiya-cho, Tsu, Mie 514-8507, Japan.
Breeding science
|December 18, 2023
概括
在小麦等Triticeae作物中,cleistogamy或闭花可以减少真菌感染. 在小麦中APETALA2 (AP2) 基因的突变减少了小体的大小,并可能导致紧的尖,提供了通向cleistogamy的途径.
科学领域:
- 植物遗传学和育种.
- 提高作物质量 提高作物质量
- 分子生物学分子生物学
背景情况:
- 在大麦等农作物中,密集化 (封闭式施肥) 可以降低真菌感染的风险.
- 麦基因 cly1,APETALA2 (AP2) 的突变,通过影响枝的发育,导致结晶.
- 在六倍体小麦 (Triticum aestivum) 中,没有自然观察到cleistogamy.
研究的目的:
- 为了识别可能诱导cleistogamy的小麦AP2ortologs中的突变.
- 为了研究AP2突变对六倍体小麦花和发展的影响.
主要方法:
- 乙基甲硫酸盐 (EMS) 在小麦中诱导的突变发生.
- 高分辨率融 (HRM) 分析以选AP2突变.
- 对AP2突变系的表型分析.
主要成果:
- 与野生类型相比,小麦AP2突变体显示了较小的体大小.
- 在D基因组中的特定AP2突变导致了紧的尖峰表型,类似于大麦的密集尖峰.
- 在A和D基因组的AP2正义基因组中发现了突变.
结论:
- 小麦AP2的正经体在形发育和形结构中起着重要作用.
- 通过将多个AP2-同源基因中的突变结合起来,可以实现生成单双性六合体小麦.
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