氧化和奥里扎林对"41B"和"Fercal"葡萄树根茎苗木的突变效应
1Department of Horticulture, Faculty of Agriculture, Selcuk University, Konya, 42250, Turkey.
Breeding science
|December 18, 2023
概括
奥里扎林和氧化物 (N2O) 有效地诱导了葡萄藤根茎中的多体化. 这些变异原体改变了植物形态,并通过流细胞计和染色体计数得到证实.
科学领域:
- 植物科学 植物科学
- 遗传学 是一个遗传学.
- 园艺园艺 园艺园艺
背景情况:
- 多重积分学可以增强植物中可取的特征.
- 葡萄树根茎对于葡萄种植至关重要.
- 诱导多重积分是一种改善葡萄品种的方法.
研究的目的:
- 为了研究奥里扎林和氧化物 (N2O) 在诱导41B和Fercal葡萄树根茎中的多体化疗法的有效性.
- 评估不同剂量和不同暴露时间对这些变异原体的影响.
- 通过形态学,细胞学和分子方法来确认多化.
主要方法:
- 在不同剂量和暴露时间下应用奥里扎林和N2O.
- 对幼苗进行形态,宏观和显微镜检查.
- 流细胞计 (FC) 分析用于DNA含量验证.
- 在体外繁殖和染色体计数用于确认性.
主要成果:
- 多种植苗的叶子厚度,叶绿素含量,口腔体大小和叶绿体数量增加,口腔体密度降低.
- 流细胞计验证了1个四倍体和4个混合体Fercal苗,以及1个混合体41B苗.
- 与二倍体相比,核DNA含量在四倍体中增加了2.00倍,在混合体中增加了1.34倍.
- 染色体计数证实,四形Fercal后代的数量增加了2倍.
结论:
- 奥里扎林和N2O是诱导葡萄藤根茎中多体积的有效变异原体.
- 建议结合流细胞计和染色体计数的使用,以准确确认ploidy水平.
- 在这些根茎中诱导的多体性显示出改善葡萄藤特性的潜力.
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