开发一种快速,灵敏和选择性的LC-MS/MS方法来量化健康人体尿中的黄素水平:胡对黄素生物可用性的影响
Sana Khajeh Pour1, Cynthia Blanton2, Biwash Ghimire1
1Department of Biomedical and Pharmaceutical Sciences Idaho State University Pocatello Idaho USA.
Food science & nutrition
|December 18, 2023
概括
在黑胡中发现的皮佩林显著提高了黄素 (CCM) 的生物可用性. 与piperine的同时摄入增加了CCM的半衰期和尿液分泌,改善了吸收和减少了清除.
科学领域:
- 药理动力学和药物新陈代谢
- 自然产品化学 自然产品化学
- 分析化学 分析化学
背景情况:
- 黄 (CCM) 是一种来自黄的化合物,已知有健康益处,但由于新陈代谢快速,其口服生物可用性较低.
- 提高CCM的生物可用性对于最大限度地发挥其治疗潜力在治疗炎症和代谢疾病方面至关重要.
研究的目的:
- 开发和验证一种敏感的LC-MS/MS测定方法,用于量化人体尿中的黄素 (CCM).
- 为了研究黑胡成分皮佩林对健康志愿者的CCM口服吸收和生物可用性的影响.
主要方法:
- 开发一种选择性,灵敏和强大的LC-MS/MS方法,用于尿液中CCM量化 (线性范围为0.62540 ng/mL).
- 一项交叉研究,健康的志愿者在24小时内收集尿液样本,与黑胡或没有黑胡一起食用CCM.
- 分析CCM在尿液中的分泌,以评估生物可用性和药理学参数.
主要成果:
- 同时摄入皮佩林显著增加了CCM半衰期,从2.2 ± 0.79小时增加到4.5 ± 0.80小时.
- 与单独使用CCM (49.45 ± 12.94μg) 相比,与 piperine (218.14 ± 94.98μg) 联合使用时,CCM在24小时内通过尿液的分泌量显著更高.
- 开发的LC-MS/MS方法表现出极好的线性,灵敏度 (LLOQ 0.625 ng/mL) 和特异性.
结论:
- 皮皮林显著提高了黄素 (CCM) 的口服吸收和生物可用性.
- 与piperine同时使用会降低CCM的全身清除.
- 这项研究提供了证据支持使用皮佩林来提高CCM的治疗疗效.
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