通过聚烯酸的衍生来改善MALDI-TOFMS的电离效率和结构分析的应用
Masahiro Hashimoto1, Haruo Iwabuchi1, Takaya Satoh1
1JEOL Ltd., 3-1-2 Musashino, Akishima, Tokyo 196-8558, Japan.
Mass spectrometry (Tokyo, Japan)
|December 18, 2023
概括
矩阵辅助激光脱/电离飞行时间质谱法 (MALDI-TOFMS) 有效地分析了聚合物分子重量分布. 衍生物化,像甲基化一样,增强了对具有挑战性的聚合物,如聚烯酸的检测,使得准确的聚合度的确定.
科学领域:
- 聚合物化学 聚合物化学
- 分析化学 分析化学
- 质谱测量质量谱测量
背景情况:
- 矩阵辅助激光脱/离子化飞行时间质谱法 (MALDI-TOFMS) 是一种用于聚合物分析的强大技术.
- MALDI是一种软电离方法,通常产生单电荷离子,简化了分子重量分布分析.
- 某些聚合物,如聚烯酸及其碳酸组的聚烯酸,具有较低的电离效率,阻碍了准确的MALDI-TOFMS检测.
研究的目的:
- 引入一种衍生方法,以提高聚合物的电离效率,用于MALDI-TOFMS分析.
- 用精确的质谱测量来确定具有挑战性的聚合物的聚合度.
- 通过并联飞行时间质谱测量来估计聚合物的终端组结构.
主要方法:
- 使用衍生技术,特别是甲基化,以提高聚烯酸的电离效率.
- 采用精确的质谱仪来精确确定聚合物的聚合度.
- 应用双重飞行时间质谱法 (MS/MS) 来阐明聚合物末端组的结构.
主要成果:
- 通过甲基化成功提高了聚烯酸的电离效率.
- 精确确定以前难以分析的聚合物的聚合度.
- 估计聚合物终端组结构,提供进一步的分子洞察力.
结论:
- 衍生是克服特定聚合物类型的MALDI-TOFMS中电离化挑战的可行策略.
- 精确的质谱与MALDI-TOFMS和衍生相结合,为全面的聚合物表征提供了强大的方法.
- 协同MS提供了关于聚合物终端组结构的宝贵信息,补充了分子量分析.
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