UDP-葡萄糖/P2Y14受体轴促进了依赖于埃索诺菲尔的大肠炎症
Li Liu1, Takayoshi Ito1, Bo Li1
1Department of Microbiology and Immunology, Laboratory of Immune Regulation, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.
尿素二酸葡萄糖通过通过P2Y14受体增加乙酸氨基细胞来促进性结肠炎 (UC) 炎症. 阻止这种途径可以减少结肠炎的严重程度,为UC提供潜在的治疗点.
科学领域:
- 胃肠病学 胃肠病学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 性结肠炎 (UC) 是一种慢性炎症性肠病,其全球发病率正在增加.
- 在结肠中埃索诺菲尔的积累与UC疾病活性相关.
- 在UC中驱动肠道叶绿素的分子机制尚未完全理解.
研究的目的:
- 调查尿素二酸葡萄糖 (UDP-葡萄糖) 和P2Y14受体在促进UC中的结肠炎症和eosinophilia中的作用.
- 阐明涉及UDP-葡萄糖介导的乙氨基酸激活和生存的分子途径.
主要方法:
- 在UC患者的结肠粘膜中分析P2RY14mRNA表达.
- 在小鼠模型中测量UDP-葡萄糖水平,用于测量德克斯硫酸 (DSS) 诱导的大肠炎.
- 在P2ry14缺乏和P2Y14受体阻塞的小鼠中评估结肠炎的严重程度.
- 骨髓移植实验使用野生类型和P2ry14缺乏的乙氨基酸.
- 研究P2Y14受体信号通路 (ERK1/2) 在乙酸性细胞中.
主要成果:
- 在UC患者的结肠粘膜中,P2RY14mRNA被上调.
- 在DSS诱导的大肠炎小鼠模型中,UDP-葡萄糖水平升高.
- P2ry14缺乏和P2Y14受体阻塞减弱了DSS诱导的大肠炎.
- 在大肠中具有高表达的P2ry14 mRNA.
- 通过P2Y14/ERK1/2信号传递,UDP-葡萄糖延长了乙氨基细胞的寿命,并促进了基因转录,加剧了结肠炎.
结论:
- 在UDP-葡萄糖/P2Y14受体轴促进结肠炎症在UC通过增强乙氨基酸细胞的积累和激活.
- 向UDP-葡萄糖/P2Y14通路代表了性结肠炎的潜在治疗策略.
更多相关视频
07:39Author Spotlight: Investigating the Effects of Compounds on Intestinal Tissue Using 3D Human Cell Line Models
Published on: September 1, 2023
06:57Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
Published on: September 18, 2016
相关概念视频
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
Peptic Ulcer Disease I: Introduction
An acute ulcer, marked by superficial erosion and minimal inflammation, swiftly resolves upon identifying and addressing the underlying cause. In contrast, a chronic ulcer persists, potentially eroding through the muscular wall and forming fibrous tissue.
Peptic ulcers can also be...
Pathophysiology of Peptic Ulcer Disease: Injurious Factors
In the antrum region, G cells secrete the gastrin hormone that binds to gastrin-cholecystokinin-B (CCK2) receptors on parietal and enterochromaffin-like (ECL) cells in the fundic glands. Simultaneously, the vagus nerve releases acetylcholine, which binds...
Glucose Absorption Into the Small Intestine
Pathophysiology of Peptic Ulcer Disease: Mucosal Defense Factors
Gastritis-II: Pathophysiology
In acute gastritis, the gastric mucosa becomes swollen and red and undergoes superficial erosion. Superficial ulceration may lead to bleeding.
In chronic gastritis, persistent or repeated insults lead to chronic inflammatory changes and, eventually, thinning or atrophy of the gastric tissue.
Gastritis can stem from various causes, each...
