对肉类供应链安全性中的菌态的LC-MS/MS分析
Claudia Ancillotti1, Lisa Bonciani1, Davide Passerini1
1Biochemie Lab S.r.l., Via di Limite 27G, Campi Bisenzio (FI), 50013, Italy.
Journal of mass spectrometry : JMS
|December 18, 2023
概括
对肉类中的菌态的监测对于动物健康和消费者安全至关重要. 一种新的LC-MS/MS方法有效地检测到这些在肉制品中的料添加剂,而不需要复杂的净化.
科学领域:
- 食品化学 食品化学
- 分析化学 分析化学
- 兽医药理学 兽医药理学
背景情况:
- 菌静止剂是获准的料添加剂,用于预防生产食品动物的菌病.
- 这些物质,包括离子体和合成分子,可以存在于肉制品中.
- 肉类矩阵含有诸如蛋白质,脂质和脂质等干扰化合物,使分析复杂化.
研究的目的:
- 开发和验证一种敏感和选择性的LC-MS/MS方法,用于肉类中的菌体分析.
- 评估商业肉制品中菌定位剂的存在和含量.
- 通过监测食品供应链中的菌剂残留物来确保消费者的健康.
主要方法:
- 使用高性能液体色谱与双重质谱法 (HPLC-MS/MS) 结合使用.
- 开发了一种简化的分析程序,省略了固态提取 (SPE) 净化步骤.
- 用矩阵匹配校准来准确量化分析物.
- 方法验证包括使用尖端样本进行回收,可重复性和不确定性评估.
主要成果:
- 开发的LC-MS/MS方法在肉类中检测出高选择性和敏感性.
- 对来自和子的真肉和肝脏样本的分析检测到非离子体的菌态.
- 量化度低于确定的最大残留量限制 (MRL).
结论:
- 简化LC-MS/MS方法对于肉制品的常规球体监测是有效的.
- 即使低于MRL的菌静止剂的存在,也强调了持续监测的重要性.
- 在肉类供应链中持续监测菌定位剂对于保护公众健康至关重要.
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