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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
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多波结构化基于照明的光学衍射断层扫描.

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    这项研究引入了基于多波结构照明的光学衍射断层扫描 (MHSI-ODT) 以实现更快的3D折射率成像. MHSI-ODT提高了成像速度16.7%,并提高了透明样品的强度利用率.

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    科学领域:

    • 光学成像技术的使用.
    • 断层扫描 (Tomography) 是一个专业的技术.
    • 生物物理学的生物物理.

    背景情况:

    • 3D折射率 (RI) 成像面临着成像速度和空间分辨率之间的权衡.
    • 传统的光衍射断层扫描 (ODT) 方法在实现高速和分辨率方面都有局限性.

    研究的目的:

    • 介绍一项新的基于光照的多波结构光学衍射断层扫描 (MHSI-ODT) 技术.
    • 为了提高成像速度和强度利用在透明样品的3DRI映射.

    主要方法:

    • 使用数字微镜装置 (DMD) 产生多声波结构化照明.
    • 通过从相移全息图解出光谱冠来获得3D RI地图.
    • 采用一级和二级波来提高照明强度的利用率.

    主要成果:

    • 与传统的SI-ODT相比,MHSI-ODT可以提高16.7%的成像速度.
    • 证明了对聚乙烯微球和生物样本的3D RI分布进行染的能力.
    • 实现了结构化照明的更好的强度利用.

    结论:

    • 在3D折射率成像速度和效率方面,MHSI-ODT提供了显著的进步.
    • 该技术有效用于成像各种透明样品,包括微粒和生物标本.