使用膨胀显微镜的Plasmodium falciparum内红细胞发育的地图
Benjamin Liffner1, Ana Karla Cepeda Diaz2,3, James Blauwkamp1
1Department of Pharmacology and Toxicology, Indiana University School of Medicine, Indianapolis, United States.
eLife
|December 18, 2023
概括
超结构膨胀显微镜揭示了疟疾寄生虫Plasmodium falciparum的3D组织. 这种方法详细介绍了无性血液发育阶段的器官生物发生和细胞生物学.
科学领域:
- 细胞生物学 细胞生物学
- 寄生虫学的寄生虫学
- 显微镜的使用方法
背景情况:
- 包括Plasmodium falciparum在内的虫复杂寄生虫表现出多样化的细胞生物学,但它们的小尺寸挑战了光显微镜研究.
- 超结构膨胀显微镜 (U-ExM) 通过物理扩展样本以实现详细可视化提供了一个解决方案.
研究的目的:
- 在无性血液阶段,将U-ExM应用于人类疟疾寄生虫Plasmodium falciparum.
- 阐明P. falciparum有机体的三维组织和基本的细胞生物学.
主要方法:
- 超结构膨胀显微镜 (U-ExM) 对P. falciparum的应用.
- 使用染料结合试剂和免疫染料来对细胞结构进行分类.
主要成果:
- 在红细胞内发育过程中目录了13种不同的P. falciparum结构和器官.
- 描述了外侧中心状板在线粒分裂过程中定细胞核中的作用.
- 详细介绍了罗普特里斯,戈尔吉,基底复合体和内膜复合体与外心状斑块的协调分离.
- 在细胞动力学过程中观察到线粒体和质细胞的连续裂变事件,与外侧心状斑块相关.
结论:
- 这项研究提供了迄今为止P. falciparum内红细胞发育的最详细的超结构分析.
- 揭示了人们对P. falciparum有机体生物发生和基本细胞生物学的不太了解的方面.
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