固相提取与感应受体的功能化在磁性微球上作为亲和力探针可以从草药提取物中选择性地捕获连接物
Peixuan Cheng1, Kaili Meng1, Xiangang Shi1
1Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, 710069, People's Republic of China.
Mikrochimica acta
|December 18, 2023
概括
我们开发了一种快速的磁性方法来固定蛋白质,用于药物发现. 这种技术成功地从草药中识别了药物联体,展示了其对天然产品查的潜力.
科学领域:
- 生物化学 生物化学
- 分析化学 分析化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 蛋白质功能化的磁性材料为从复杂的自然产品中发现药物提供了优势.
- 需要有效的方法来固定蛋白质,而无需广泛的净化.
研究的目的:
- 开发一种快速的方法,将基脱酶 (Halo) 标记的感受受体 (CaSR) 固定在磁显微球 (MM) 上,直接从细胞溶解物中获取.
- 描述neomycin在CaSR功能化的MM上的吸附特性,并选来自Radix Astragali (RA) 的配体.
主要方法:
- 从原始细胞溶解物直接将Halo标记的CaSR固定在MM上.
- 使用异热定位热量计和特姆金模型对胺素吸附的表征.
- 从Radix Astragali提取物中选配体,使用HPLC-MS.
主要成果:
- 在30分钟内成功固定了43.87mg CaSR/g MM,稳定了4周.
- 确定了neomycin吸附能力 (4.703.96 × 10−4 mol/g) 和确定了静电力作为驱动相互作用.
- 鉴定出阿斯特拉加洛西德IV,奥诺宁和卡利科辛是来自Radix Astragali提取物中的配体.
结论:
- 开发的CaSR功能化的MM作为一种有效的亲和探测器,用于识别激动剂和从复杂的自然矩阵中选择性捕获连接物.
- 该方法为药物发现和天然产品查提供了快速有效的方法.
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