相关实验视频

Updated: Jul 14, 2026

Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs

Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs

Published on: April 9, 2021

反转录循环介导的同热放大 (RT-LAMP) 原始设计基于印度尼西亚SARS-CoV-2RNA序列

Irsyad Ibadurrahman^1,2, Suryani¹, Desriani³

¹Biochemistry Department, IPB University, Jl. Raya Dramaga, Babakan, Bogor, West Java, 16680, Indonesia.

Journal, genetic engineering & biotechnology

|December 18, 2023

概括

研究人员开发了一套特定的反转录循环介导同热放大 (RT-LAMP) 启动套件,T1_9,用于跟踪SARS-CoV-2. 这本书准确地识别了来自印度尼西亚和全球的COVID-19变种,有助于疫情监测.

科学领域:

病毒学病毒学
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

由于COVID-19的流行,需要有效的病例追踪方法.
反转录循环介导同热放大 (RT-LAMP) 提供了一种快速,简单和具有成本效益的方法.
没有RT-LAMP原始设计专门使用印度尼西亚SARS-CoV-2基因组序列.

研究的目的:

设计和验证使用来自印度尼西亚和全球不同地区的SARS-CoV-2基因组序列的RT-LAMP基础.
在原始设计过程中包括来自五种SARS-CoV-2关注变种 (VOC) 的基因组序列.

主要方法:

从70个SARS-CoV-2基因组生成了一个共识序列.
进行了遗传学和SimPlot分析以评估遗传多样性.
设计了139个RT-LAMP原料套,并评估了它们的特性.
在候选原料上进行二次结构和特异性测试 (BLAST NCBI,eLAMP BLAST).

主要成果:

建立了29982个基的共识序列,与SARS-CoV-2武汉分离物密切相关.
在病毒基因组的特定区域观察到高遗传多样性.
四个基础套件 (T1_6,T1_9,T4_7,T4_52) 呈现出最佳的特性.
原料组T1_9表现出优越的特异性,并且在工作温度下避免了二次结构的形成.

结论:

鉴于T1_9原料套件是理想的,满足了所有参数,具有出色的特异性.
这款初始套件推用于未来的SARS-CoV-2检测工作.
T1_9原料可以有效地识别来自印度尼西亚,其他大陆的SARS-CoV-2以及包括Omicron亚型在内的关键VOC.

关键词:

在 COVID-19 疫情中,初级设计设计初级设计初级设计土耳其人 RT-LAMP 这就是SARS-CoV-2病毒.

更多相关视频

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

相关概念视频

PCR

PCR

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

RACE - Rapid Amplification of cDNA Ends

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...