在Citrobacter rodentium肠道感染期间,TRPV1控制天生的免疫力
Michael Cremin1, Emmy Xue Yun Tay1, Valerie T Ramirez1
1Department of Anatomy, Physiology and Cell Biology, UC Davis School of Veterinary Medicine, UC Davis, Davis, California, United States of America.
PLoS pathogens
|December 18, 2023
概括
暂时受体潜在的化物受体1 (TRPV1) 在控制肠道细菌感染方面发挥着至关重要的作用. 失去TRPV1会影响中性粒细胞的招募,增加病原体负担和肠道损伤.
科学领域:
- 免疫学 免疫学 免疫学
- 神经科学是一个神经科学.
- 微生物学 微生物学
背景情况:
- 粘膜免疫对于宿主防御肠道病原体至关重要.
- 神经元通信显著影响免疫反应.
- 表达过渡受体潜在化物受体1 (TRPV1) 的感觉神经元与宿主保护反应有关.
研究的目的:
- 研究TRPV1在协调宿主对肠道病原体Citrobacter rodentium的反应中的特定作用.
- 评估TRPV1缺乏对细菌负担,宿主病理和免疫细胞招募的影响.
主要方法:
- 使用了Citrobacter rodentium感染的小鼠模型.
- 与野生型 (WT) littermates 的TRPV1淘汰赛 (TRPV1-/-) 小鼠进行比较.
- 分析了细菌负载,结肠组织病理学和免疫细胞群 (T细胞,中性粒细胞) 以及化学因子和粘附分子的表达.
主要成果:
- 在TRPV1-/-小鼠中,C. rodentium负担显著增加,结肠密室增大.
- 在TRPV1-/-小鼠中,对结肠的中性粒细胞招募显著减少.
- 在TRPV1-/-小鼠中,化学基因Cxcl6和粘附分子Icam1的表达下降,影响内皮细胞粘附分子.
结论:
- TRPV1对于对肠道细菌病原体的有效宿主保护反应至关重要.
- TRPV1信号传递对于中性粒细胞的招募和控制肠道病原体负载至关重要.
- 这些发现突出了TRPV1在调节粘膜免疫反应对感染的作用.
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