麦格纳波特菌效应剂MoSPAB1直接激活米Bsr-d1的表达,以促进病变发生
Ziwei Zhu1,2, Jun Xiong1, Hao Shi1
1State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
Nature communications
|December 18, 2023
概括
菌病原体Magnaporthe oryzae使用MoSPAB1效应器来激活米Bsr-d1,帮助感染. 这种保存的机制,涉及促进体结合和竞争,在其他真菌中发现,揭示了病变发生的共同策略.
科学领域:
- 植物病原体相互作用
- 分子植物病理学 分子植物病理学
- 菌类基因组学 菌类基因组学
背景情况:
- 菌类病原体分泌作用因子,抑制宿主免疫力.
- 对于真菌效应因子的证据是稀缺的,它们可以通过主体防御抑制基因来激活.
- 玛格纳波特小虫感染包括米的诱导 Bsr-d1.1.
研究的目的:
- 为了确定导致大米Bsr-d1.1.的真菌效应因子.
- 为了阐明由真菌效应器诱导Bsr-d1的机制.
- 调查相关的真菌作用因子的保存和功能.
主要方法:
- 酵母的一种混合测试检测DNA结合活性.
- 电泳运动转移试验 (EMSA) 证实促进体结合.
- 位点定向的突变发生,以确定MoSPAB1.1.中的关键氨基酸.
- 在异质系统中的表达分析 (果和).
主要成果:
- 效应物MoSPAB1直接与Bsr-d1促进体结合,诱导其表达.
- MoSPAB1 的 103-123 个氨基酸对于 Bsr-d1 促进体结合至关重要.
- MoSPAB1和米的转录因子MYBS1在Bsr-d1促进体结合方面进行竞争.
- 在Colletotrichum物种中同源的SPAB1效应体激活植物Bsr-d1同源体,促进病变发生.
结论:
- MoSPAB1是一种新型的真菌效应剂,可激活宿主基因表达以促进病变发生.
- MoSPAB1-Bsr-d1相互作用代表了在真菌病原体中保存的机制.
- 这个保存的模块为控制植物真菌病提供了潜在的目标.
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