来自 Fabaceae 的前化异黄,用于抵抗 Staphylococcus aureus 中的 NorA 排泄
Marina Ika Irianti1,2, Jean-Paul Vincken1, Sarah van Dinteren1
1Laboratory of Food Chemistry, Wageningen University and Research, Bornse Weilanden 9, 6708 WG, Wageningen, The Netherlands.
Scientific reports
|December 18, 2023
概括
来自豆类的前化异黄,如新和玻璃烯,抑制了S. aureus中的NORA排泄. 这些化合物增强诺基诺对抗耐药菌株的有效性,没有显著的毒性.
科学领域:
- 微生物学 微生物学
- 药理学 药理学是指药理学的学科.
- 自然产品 化学 化学
背景情况:
- 诺拉的排水有助于 Staphylococcus aureus 的多药性耐药性.
- 诺基诺耐药性是一个日益严重的公共卫生问题.
- 自然化合物为抗微生物药物开发提供了潜在的替代品.
研究的目的:
- 调查前化异黄作为潜在的排泄抑制剂 (EPI) 对抗诺基诺耐药的黄金色杆菌.
- 为了识别抑制NORA排泄的特定的化异黄.
- 评估这些化合物作为抗性修饰剂的疗效和安全性.
主要方法:
- 选前化异黄的排泄抑制活性.
- 在NorA中评估乙积累,以评估过度表达黄金色杆菌.
- 确定化合物对诺基诺隆最小抑制度 (MIC) 的影响.
- 在Caco-2细胞中评估细胞毒性和膜透性.
主要成果:
- 四种先化异类黄,包括新化异和格拉布伦,证明了NORA排泄的抑制.
- 新巴瓦伊索弗拉和格拉布伦显著增加了乙积累,并提高了诺基诺MICs.
- 活动被证实是NORA依赖的,因为在NORA淘汰菌株中没有发现增强.
- 新巴瓦伊索弗拉的疗效与保留素相比或更高,细胞毒性较低.
结论:
- 化异黄,特别是新异黄和格拉布伦,是S. aureus中NORA排泄的有效抑制剂.
- 这些植物化学物质显示出作为抗微生物剂和抵抗修饰剂的承诺,用于对抗抗诺基诺耐药的黄金色菌.
- 新巴瓦伊索弗拉和格拉布伦具有良好的安全性,没有观察到哺乳动物细胞中细胞毒性或膜破坏.
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