古代物种中的染色体结构自然缺乏染色体蛋白质的结构维护

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Catherine Badel^1,2, Stephen D Bell^3,4

¹Molecular and Cellular Biochemistry Department, Indiana University, Bloomington, IN, USA. catherine.m.badel@gmail.com.

Nature microbiology

|December 19, 2023

概括

染色体结构维护 (SMC) 蛋白质在一些古物中不存在. 这项研究揭示了Aeropyrum pernix中独特的两部分基因组组织,这表明一种古代的染色体结构形式.

科学领域:

微生物学微生物学
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

染色体结构维护 (SMC) 蛋白质对于大多数生物体的染色体组织至关重要.
在Crenarchaeota中的Desulfurococcales系中,SMC蛋白显著缺席.
了解SMC缺陷古生物中的染色体组织对于进化见解至关重要.

研究的目的:

研究Aeropyrum pernix中的染色体组织,这是Desulfurococcales的一个模型生物.
在没有SMC蛋白质的情况下识别基因组组织的新机制.
探索不同考古系的基因组架构之间的进化关系.

主要方法:

在Aeropyrum pernix.上进行了染色体构造捕获 (3C) 实验.
对染色体相互作用域及其边界的分析.
域边界与转录和翻译活动的相关性.

主要成果:

Aeropyrum pernix表现出染色体相互作用域,类似于其他古物.
域边界取决于转录和翻译.
确定了一种更高阶的,两部分的基因组组织,具有独特的高基因表达,自我相互作用的域.
这个域的特点是转录活动和特定的循环结构.

结论:

在Aeropyrum pernix中,基因组组织是独特的,不依赖于SMC蛋白.
观察到的两部分组织可能代表了其他古生物中发现的更复杂的基因组架构的进化前体,如Sulfolobus.
这一发现揭示了不同考古遗传谱系对基因组管理的多样化策略.

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