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介酶体 stromal 细胞中的 PD-L1 表达水平预测它们对自身免疫性肝炎的治疗价值

Xilong Bai^1,2,3, Tingwei Chen¹, Yuqi Li¹

¹State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500, Yunnan, China.

Stem cell research & therapy

|December 19, 2023

概括

介酶体 stromal 细胞 (MSCs) 显示出治疗自身免疫性肝炎的前景,但它们的变异性是一个挑战. 研究人员发现,沃顿的编程死亡联结1 (PD-L1) 表达水平在沃顿.

科学领域:

免疫学免疫学免疫学
肝病学肝病学是一种肝病学.
再生医学是一种再生医学.

背景情况:

自免疫性肝炎是一种慢性肝病,缺乏有效的治疗方法.
介酶体 stromal 细胞 (MSCs) 提供治疗潜力,但表现出显著的异质性.
MSC的异质性使其在自身免疫性疾病中的临床应用变得复杂.

研究的目的:

为了研究沃顿的果衍生MSCs (WJ-MSCs) 的异质性.
确定用于预测WJ-MSC免疫调节能力的生物标志物.
评估WJ-MSCs在自身免疫性肝炎中具有变化的PD-L1表达的治疗疗效.

主要方法:

在cGMP条件下从58名人类捐赠者中分离WJ-MSC.
使用转录组和单细胞RNA测序 (scRNA-seq) 分析了WJ-MSC基因表达.
在自免疫性肝炎的小鼠模型中评估免疫调节活性和治疗效果,使用PD-L1表达作为查指标.

主要成果:

鉴定了六个不同的WJ-MSC基因表达特征集群,其中有扩散,分化,摄食因子分泌和免疫调节的变化.
通过scRNA-seq.发现了四个WJ-MSC亚群.
在PD-L1表达和WJ-MSC免疫抑制能力之间显示出正相关性;高的PD-L1WJ-MSC在自身免疫性肝炎模型中显示出优越的治疗效果.

结论:

PD-L1表达是选择最佳MSC治疗自身免疫性疾病的可靠指标.
这些发现增强了MSC的质量控制策略.
该研究为改善MSCs在临床环境中的治疗益处提供了见解.

关键词:

自体免疫性肝炎是一种自身免疫性肝炎.细胞异质性细胞异质性免疫调节免疫调节介质细胞层细胞是介质细胞层细胞.在PD-L1中.

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