武志圣补充剂对LPS诱导的肠炎和肉屏障损伤的作用
Han Sun1, Xirui Zheng1, Bowen Yang1
1College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Frontiers in veterinary science
|December 19, 2023
概括
武志圣 (WZS) 保护肉免受肠道炎症和由脂多糖 (LPS) 压力引起的屏障损伤. 这种传统的中国配方通过抑制NF-κB/TLR4/MyD88通路来减少炎症标志物并改善肠道屏障功能.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 动物营养 动物营养
- 免疫学 免疫学 免疫学
背景情况:
- 肠道炎症和屏障损伤会损害营养吸收,并可能导致慢性疾病.
- 脂聚糖化物 (LPS) 是一种常见的内毒素,会诱导肉的肠道炎症和屏障功能障碍.
- 传统的中国草药配方Wu Zhi San (WZS) 正在研究其对胃肠道健康的潜在治疗作用.
研究的目的:
- 评估WZS对脂聚糖 (LPS) 诱导的肠道炎症和肉屏障损伤的保护作用.
- 调查潜在的分子机制,包括NF-κB/TLR4/MyD88信号通路,通过这些信号通路,WZS可以发挥其保护作用.
主要方法:
- 总共有200只肉被分为五组:对照组,LPS诱导的压力组和三个WZS治疗组 (高剂量,中剂量,低剂量).
- 在两个不同的压力阶段进行了LPS挑战.
- 通过测量血清标记物 (MPO,DAO,SOD,D-LA),阴形态 (VH,CD),炎症性细胞因子表达 (TNF-α,IL-1β,IL-6,IL-10) 和关键信号通路蛋白质 (NF-κB,TLR4,MyD88,Claudin-1,Occludin-1,ZO-1) 来评估肠道保护.
主要成果:
- 补充WZS显著降低了血清MPO,DAO和D-LA水平,同时增加了SOD水平.
- WZS改善了阴形态,通过增加高度 (VH) 和减少密室深度 (CD) 表示.
- WZS显著降低了NF-κB,TLR4,MyD88和促炎性细胞因子 (TNF-α,IL-1β,IL-6) 的表达,并上调了紧结蛋白 (Claudin-1,Occludin-1,ZO-1).
结论:
- 用WZS的食补充剂有效地减轻了受LPS压力的 brojlers的肠道炎症和屏障损伤.
- WZS通过抑制NF-κB/TLR4/MyD88信号通路来发挥其保护作用,从而减少炎症反应并增强肠道屏障完整性.
- 在家禽生产中,WZS代表了一种有前途的治疗药物,用于治疗胃肠道疾病.
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