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质量细胞计检测可检测的HaloTag连接体设计参数

Nicole Potter1, Simon Latour2,3,4, Edmond C N Wong1

  • 1Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Ontario M5S 3H6, Canada.

Bioconjugate chemistry
|December 19, 2023
PubMed
概括

研究人员为HaloTag开发了新的质量细胞测量配体,使生物样本的高维分析成为可能. 这些配体促进了强大的细胞标记,化减少了非特异性结合,从而改善了细胞分辨能力.

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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